Control of the expression of c-sis mRNA in human glioblastoma cells by phorbol ester and transforming growth factor β

Richard Press, A. Misra, G. Gillaspy, D. Samols, D. A. Goldthwait

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The regulation of c-sis oncogene expression in human gliosblastoma cell line A172 has been investigated using a sensitive RNA-RNA solution hybridization method. Enhanced expression of c-sis mRNA was induced by phorbol ester (PMA) and diacylglycerol, each of which activates protein kinase C. c-sis mRNA was also induced by transforming growth factor β (TGF-β). The response to PMA and TGF-β was transient, and in each case the decrease in c-sis mRNA level following maximum stimulation occurred with a half-life similar to the mRNA half-life previously determined. Cycloheximide had no significant effect on the induction of c-sis mRNA by either PMA or TGF-β. The increases in c-sis mRNA following addition of either PMA or TGF-β correlated well with increases in c-sis transcription as observed by the nuclear run-on technique. In cells in which protein kinase C had been down-regulated, there was no inhibition of the c-sis mRNA response to TGF-β. Furthermore in cells pretreated with TGF-β, induction by PMA was unaffected. Thus the TGF-β signal pathway does not involve activation of protein kinase C, and at least two initially distinct intracellular signaling routes lead to activation of c-sis gene expression in this glioblastoma cell line. The protein kinase inhibitor H7 abolished the ability of not only PMA but also of TGF-β to induce c-sis mRNA. The ability of H7 to inhibit the TGF-β stimulation suggests that a protein kinase other than protein kinase C is involved in the signal transduction by TGF-β.

Original languageEnglish (US)
Pages (from-to)2914-2920
Number of pages7
JournalCancer Research
Volume49
Issue number11
StatePublished - 1989
Externally publishedYes

Fingerprint

Transforming Growth Factors
Phorbol Esters
Glioblastoma
Messenger RNA
Protein Kinase C
sis Genes
Half-Life
Signal Transduction
RNA
Cell Line
Diglycerides
Cycloheximide
Protein Kinase Inhibitors
Protein Kinases
Gene Expression

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Control of the expression of c-sis mRNA in human glioblastoma cells by phorbol ester and transforming growth factor β. / Press, Richard; Misra, A.; Gillaspy, G.; Samols, D.; Goldthwait, D. A.

In: Cancer Research, Vol. 49, No. 11, 1989, p. 2914-2920.

Research output: Contribution to journalArticle

Press, R, Misra, A, Gillaspy, G, Samols, D & Goldthwait, DA 1989, 'Control of the expression of c-sis mRNA in human glioblastoma cells by phorbol ester and transforming growth factor β', Cancer Research, vol. 49, no. 11, pp. 2914-2920.
Press, Richard ; Misra, A. ; Gillaspy, G. ; Samols, D. ; Goldthwait, D. A. / Control of the expression of c-sis mRNA in human glioblastoma cells by phorbol ester and transforming growth factor β. In: Cancer Research. 1989 ; Vol. 49, No. 11. pp. 2914-2920.
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