Control of neurosecretion in the moth Manduca sexta: Physiological regulation of the eclosion hormone cells

Philip Copenhaver, James W. Truman

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

1. Metamorphosis in the moth Manduca sexta culminates with the secretion of the peptide eclosion hormone (EH), which triggers the stereotyped behavior of adult emergence (eclosion) from the pupal cuticle. In restrained but spontaneously behaving animals, the release of EH occurred shortly before the onset of subjective night (Fig. 3) and coincided with a depletion of EH from the neurohemal organs of the brain, the corpora cardiaca-corpora allata complex (CC-CA; Fig. 4). 2. EH is produced by neurons within a bilaterally paired group of brain neurosecretory cells (Group Ia) which project to the CC-CA via the nervi corporis cardiaci-1+2 (NCC-1+2; Fig. 1). Electrical stimulation of the NCC-1+2 caused a marked increase in the levels of EH secreted from isolated CC-CA (Fig. 2), while stimulation of the other nerves innervating the neurohemal organs did not. 3. Electrical activity in the NCC-1+2 paralleled that of the cerebral neurosecretory cells (Fig. 1). Chronic extracellular recordings revealed a sudden increase in the tonic firing of several units within this nerve approximately 2 to 3 h before normal eclosion (Fig. 5), coincident with the release of EH bioactivity from the CC-CA (Fig. 6). 4. The Group Ia neurons were electrically inactive on the day before eclosion (Day -1), but on the day of eclosion (Day 0) a subgroup of these cells exhibited both enhanced synaptic input and elevated rates of tonic firing during the normal time of EH release (Fig. 7). 5. No significant differences in resting membrane potential or spike waveform characteristics were detected among the various subsets of Group Ia cells on either Day -1 or Day 0, while a significant increase in the resting input resistance was seen in the active subgroup on Day 0 (Fig. 8). This increase may be due to the regulatory effects of the steroid 20-hydroxyecdysone, which inhibits the release of EH and may act by preventing the synaptic activation of the EH neurons until the final day of adult development.

Original languageEnglish (US)
Pages (from-to)445-455
Number of pages11
JournalJournal of Comparative Physiology A
Volume158
Issue number4
DOIs
StatePublished - Jul 1986
Externally publishedYes

Fingerprint

eclosion hormone
Neurosecretion
neurosecretion
Manduca
physiological regulation
Moths
Manduca sexta
moth
hormone
moths
eclosion
cells
Corpora Allata
neurosecretory cells
neurons
Neurons
brain
nerve tissue
Ecdysterone
Stereotyped Behavior

ASJC Scopus subject areas

  • Behavioral Neuroscience
  • Neuroscience(all)
  • Physiology (medical)
  • Physiology
  • Animal Science and Zoology

Cite this

Control of neurosecretion in the moth Manduca sexta : Physiological regulation of the eclosion hormone cells. / Copenhaver, Philip; Truman, James W.

In: Journal of Comparative Physiology A, Vol. 158, No. 4, 07.1986, p. 445-455.

Research output: Contribution to journalArticle

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abstract = "1. Metamorphosis in the moth Manduca sexta culminates with the secretion of the peptide eclosion hormone (EH), which triggers the stereotyped behavior of adult emergence (eclosion) from the pupal cuticle. In restrained but spontaneously behaving animals, the release of EH occurred shortly before the onset of subjective night (Fig. 3) and coincided with a depletion of EH from the neurohemal organs of the brain, the corpora cardiaca-corpora allata complex (CC-CA; Fig. 4). 2. EH is produced by neurons within a bilaterally paired group of brain neurosecretory cells (Group Ia) which project to the CC-CA via the nervi corporis cardiaci-1+2 (NCC-1+2; Fig. 1). Electrical stimulation of the NCC-1+2 caused a marked increase in the levels of EH secreted from isolated CC-CA (Fig. 2), while stimulation of the other nerves innervating the neurohemal organs did not. 3. Electrical activity in the NCC-1+2 paralleled that of the cerebral neurosecretory cells (Fig. 1). Chronic extracellular recordings revealed a sudden increase in the tonic firing of several units within this nerve approximately 2 to 3 h before normal eclosion (Fig. 5), coincident with the release of EH bioactivity from the CC-CA (Fig. 6). 4. The Group Ia neurons were electrically inactive on the day before eclosion (Day -1), but on the day of eclosion (Day 0) a subgroup of these cells exhibited both enhanced synaptic input and elevated rates of tonic firing during the normal time of EH release (Fig. 7). 5. No significant differences in resting membrane potential or spike waveform characteristics were detected among the various subsets of Group Ia cells on either Day -1 or Day 0, while a significant increase in the resting input resistance was seen in the active subgroup on Day 0 (Fig. 8). This increase may be due to the regulatory effects of the steroid 20-hydroxyecdysone, which inhibits the release of EH and may act by preventing the synaptic activation of the EH neurons until the final day of adult development.",
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