Control of brain temperature during experimental global ischemia in rats

Ansgar Brambrink, Laszlo Kopacz, Andreas Astheimer, Holger Noga, Axel Heimann, Oliver Kempski

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Temperature control during experimental ischemia continues to be of major interest. However, if exposure of brain tissue is necessary during the experiment, regional heat loss may occur even when the core temperature is maintained. Furthermore, valid non-invasive brain temperature monitoring is difficult in small rodents. This paper describes a method for both monitoring and maintenance of brain temperature during small animal preparations in a stereotaxic frame. The device used includes an ear-bar thermocouple probe and a small near-infrared radiator. The new equipment permitted to maintain peri- ischemic brain temperature at a desired level while carrying out non-invasive continuous recordings of cerebral blood flow (laser Doppler-flowmetry) and of electrical brain function (EEG). In contrast, without extracranial heat application, superficial and basal brain temperatures decreased during global cerebral ischemia by 4.1±0.1 and 4.6±0.4°C (mean±SEM), respectively, returning to baseline values at 15-30 min of reperfusion while rectal (core) temperature remained stable at baseline values. The ear-bar thermocouple probe (tympanic membrane) reliably reflected basal brain temperature, and temperature in superficial brain areas correlated well with that in the temporal muscle. Our data show that the new system allows to exclude unwanted hypothermic neuroprotection, and does not interfere with optical and electrical measurement techniques.

Original languageEnglish (US)
Pages (from-to)111-122
Number of pages12
JournalJournal of Neuroscience Methods
Volume92
Issue number1-2
DOIs
StatePublished - Oct 15 1999
Externally publishedYes

Fingerprint

Ischemia
Temperature
Brain
Ear
Cerebrovascular Circulation
Hot Temperature
Temporal Muscle
Equipment and Supplies
Laser-Doppler Flowmetry
Tympanic Membrane
Brain Ischemia
Reperfusion
Electroencephalography
Rodentia
Maintenance

Keywords

  • Brain temperature
  • Ear-bar thermocouple probe
  • Hypothermia
  • Ischemia
  • Near-infrared radiator
  • Neuroprotection
  • Rat
  • Tympanic membrane

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Brambrink, A., Kopacz, L., Astheimer, A., Noga, H., Heimann, A., & Kempski, O. (1999). Control of brain temperature during experimental global ischemia in rats. Journal of Neuroscience Methods, 92(1-2), 111-122. https://doi.org/10.1016/S0165-0270(99)00100-4

Control of brain temperature during experimental global ischemia in rats. / Brambrink, Ansgar; Kopacz, Laszlo; Astheimer, Andreas; Noga, Holger; Heimann, Axel; Kempski, Oliver.

In: Journal of Neuroscience Methods, Vol. 92, No. 1-2, 15.10.1999, p. 111-122.

Research output: Contribution to journalArticle

Brambrink, A, Kopacz, L, Astheimer, A, Noga, H, Heimann, A & Kempski, O 1999, 'Control of brain temperature during experimental global ischemia in rats', Journal of Neuroscience Methods, vol. 92, no. 1-2, pp. 111-122. https://doi.org/10.1016/S0165-0270(99)00100-4
Brambrink A, Kopacz L, Astheimer A, Noga H, Heimann A, Kempski O. Control of brain temperature during experimental global ischemia in rats. Journal of Neuroscience Methods. 1999 Oct 15;92(1-2):111-122. https://doi.org/10.1016/S0165-0270(99)00100-4
Brambrink, Ansgar ; Kopacz, Laszlo ; Astheimer, Andreas ; Noga, Holger ; Heimann, Axel ; Kempski, Oliver. / Control of brain temperature during experimental global ischemia in rats. In: Journal of Neuroscience Methods. 1999 ; Vol. 92, No. 1-2. pp. 111-122.
@article{5445e90e1c504728ade92002148b15ab,
title = "Control of brain temperature during experimental global ischemia in rats",
abstract = "Temperature control during experimental ischemia continues to be of major interest. However, if exposure of brain tissue is necessary during the experiment, regional heat loss may occur even when the core temperature is maintained. Furthermore, valid non-invasive brain temperature monitoring is difficult in small rodents. This paper describes a method for both monitoring and maintenance of brain temperature during small animal preparations in a stereotaxic frame. The device used includes an ear-bar thermocouple probe and a small near-infrared radiator. The new equipment permitted to maintain peri- ischemic brain temperature at a desired level while carrying out non-invasive continuous recordings of cerebral blood flow (laser Doppler-flowmetry) and of electrical brain function (EEG). In contrast, without extracranial heat application, superficial and basal brain temperatures decreased during global cerebral ischemia by 4.1±0.1 and 4.6±0.4°C (mean±SEM), respectively, returning to baseline values at 15-30 min of reperfusion while rectal (core) temperature remained stable at baseline values. The ear-bar thermocouple probe (tympanic membrane) reliably reflected basal brain temperature, and temperature in superficial brain areas correlated well with that in the temporal muscle. Our data show that the new system allows to exclude unwanted hypothermic neuroprotection, and does not interfere with optical and electrical measurement techniques.",
keywords = "Brain temperature, Ear-bar thermocouple probe, Hypothermia, Ischemia, Near-infrared radiator, Neuroprotection, Rat, Tympanic membrane",
author = "Ansgar Brambrink and Laszlo Kopacz and Andreas Astheimer and Holger Noga and Axel Heimann and Oliver Kempski",
year = "1999",
month = "10",
day = "15",
doi = "10.1016/S0165-0270(99)00100-4",
language = "English (US)",
volume = "92",
pages = "111--122",
journal = "Journal of Neuroscience Methods",
issn = "0165-0270",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Control of brain temperature during experimental global ischemia in rats

AU - Brambrink, Ansgar

AU - Kopacz, Laszlo

AU - Astheimer, Andreas

AU - Noga, Holger

AU - Heimann, Axel

AU - Kempski, Oliver

PY - 1999/10/15

Y1 - 1999/10/15

N2 - Temperature control during experimental ischemia continues to be of major interest. However, if exposure of brain tissue is necessary during the experiment, regional heat loss may occur even when the core temperature is maintained. Furthermore, valid non-invasive brain temperature monitoring is difficult in small rodents. This paper describes a method for both monitoring and maintenance of brain temperature during small animal preparations in a stereotaxic frame. The device used includes an ear-bar thermocouple probe and a small near-infrared radiator. The new equipment permitted to maintain peri- ischemic brain temperature at a desired level while carrying out non-invasive continuous recordings of cerebral blood flow (laser Doppler-flowmetry) and of electrical brain function (EEG). In contrast, without extracranial heat application, superficial and basal brain temperatures decreased during global cerebral ischemia by 4.1±0.1 and 4.6±0.4°C (mean±SEM), respectively, returning to baseline values at 15-30 min of reperfusion while rectal (core) temperature remained stable at baseline values. The ear-bar thermocouple probe (tympanic membrane) reliably reflected basal brain temperature, and temperature in superficial brain areas correlated well with that in the temporal muscle. Our data show that the new system allows to exclude unwanted hypothermic neuroprotection, and does not interfere with optical and electrical measurement techniques.

AB - Temperature control during experimental ischemia continues to be of major interest. However, if exposure of brain tissue is necessary during the experiment, regional heat loss may occur even when the core temperature is maintained. Furthermore, valid non-invasive brain temperature monitoring is difficult in small rodents. This paper describes a method for both monitoring and maintenance of brain temperature during small animal preparations in a stereotaxic frame. The device used includes an ear-bar thermocouple probe and a small near-infrared radiator. The new equipment permitted to maintain peri- ischemic brain temperature at a desired level while carrying out non-invasive continuous recordings of cerebral blood flow (laser Doppler-flowmetry) and of electrical brain function (EEG). In contrast, without extracranial heat application, superficial and basal brain temperatures decreased during global cerebral ischemia by 4.1±0.1 and 4.6±0.4°C (mean±SEM), respectively, returning to baseline values at 15-30 min of reperfusion while rectal (core) temperature remained stable at baseline values. The ear-bar thermocouple probe (tympanic membrane) reliably reflected basal brain temperature, and temperature in superficial brain areas correlated well with that in the temporal muscle. Our data show that the new system allows to exclude unwanted hypothermic neuroprotection, and does not interfere with optical and electrical measurement techniques.

KW - Brain temperature

KW - Ear-bar thermocouple probe

KW - Hypothermia

KW - Ischemia

KW - Near-infrared radiator

KW - Neuroprotection

KW - Rat

KW - Tympanic membrane

UR - http://www.scopus.com/inward/record.url?scp=0032755427&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032755427&partnerID=8YFLogxK

U2 - 10.1016/S0165-0270(99)00100-4

DO - 10.1016/S0165-0270(99)00100-4

M3 - Article

C2 - 10595709

AN - SCOPUS:0032755427

VL - 92

SP - 111

EP - 122

JO - Journal of Neuroscience Methods

JF - Journal of Neuroscience Methods

SN - 0165-0270

IS - 1-2

ER -