Contribution of GPR30 for 1,25 dihydroxyvitamin D 3 protection in EAE

Sandhya Subramanian, Lisa M. Miller, Marjorie R. Grafe, Arthur A. Vandenbark, Halina Offner

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Previous studies have demonstrated that vitamin D3-mediated protection in EAE occurs only in females and is dependent on the presence of diestrus levels of 17β-estradiol (E2). To evaluate the role of estrogen receptors in vitamin D3 treatment of EAE, we compared disease severity, CNS histopathology and immunological responses in vehicle and calcitrol (1,25 dihydroxyvitamin D 3) treated WT C57BL/6 mice vs. GPR30 membrane estrogen receptor (MER) knockout mice with MOG-35-55 peptide-induced EAE. Our results demonstrated that vitamin D 3-mediated prevention of clinical signs, CNS cellular lesions and demyelination observed in WT mice was abrogated in GPR30-KO mice with EAE. Regulatory effects of vitamin D 3 treatment that were MER dependent included increased levels of IL-10 and IL-6 secreted by MOG peptide-reactive splenocytes and increased expression of CCL5, CCR1 & CCR3 in spleen tissue. These results demonstrate for the first time that the MER is a key contributor to the E2-dependent effects of vitamin D 3-mediated protection in EAE.

Original languageEnglish (US)
Pages (from-to)29-35
Number of pages7
JournalMetabolic brain disease
Volume27
Issue number1
DOIs
StatePublished - Mar 1 2012

Keywords

  • 1,25 dihydroxyvitamin D
  • EAE
  • Estrogen
  • GPR30

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Neurology
  • Cellular and Molecular Neuroscience

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