TY - JOUR
T1 - Contribution of a membrane estrogen receptor to the estrogenic regulation of body temperature and energy homeostasis
AU - Roepke, Troy A.
AU - Bosch, Martha A.
AU - Rick, Elizabeth A.
AU - Lee, Benjamin
AU - Wagner, Edward J.
AU - Seidlova-Wuttke, Dana
AU - Wuttke, Wolfgang
AU - Scanlan, Thomas S.
AU - Rønnekleiv, Oline K.
AU - Kelly, Martin J.
PY - 2010/10
Y1 - 2010/10
N2 - The hypothalamus is a key region of the central nervous system involved in the control of homeostasis, including energy and core body temperature (Tc). 17β-Estradiol (E2) regulates Tc, in part, via actions in thebasalhypothalamusandpreoptic area. E2 primarily controlshypothalamicfunctions viathenuclear steroid receptors, estrogen receptor α/β. However, we have previously described an E2-responsive, Gq-coupled membrane receptor that reduces the postsynaptic inhibitory γ-aminobutyric acid-ergic tone and attenuates postovariectomy body weight gain in female guinea pigs through the administration of a selective Gq-mER ligand, STX.To determine the role of Gq-mER in regulating Tc, energy and bone homeostasis, ovariectomized female guinea pigs, implanted ip with temperature probes, were treated with STX or E2 for 7-8 wk. Tc was recorded for 4 wk,where as food intake and body weight were monitored daily. Bone density and fat accumulation were determined postmortem. Both E2 and STX significantly reduced Tc in the females compared with controls. STX, similar to E2, reduced food intake and fat accumulation and increased tibial bone density. Therefore, a Gq-mER-coupled signaling pathway appears to be involved in maintaining homeostatic functions and may constitute a novel therapeutic target for treatment of hypoestrogenic symptoms.
AB - The hypothalamus is a key region of the central nervous system involved in the control of homeostasis, including energy and core body temperature (Tc). 17β-Estradiol (E2) regulates Tc, in part, via actions in thebasalhypothalamusandpreoptic area. E2 primarily controlshypothalamicfunctions viathenuclear steroid receptors, estrogen receptor α/β. However, we have previously described an E2-responsive, Gq-coupled membrane receptor that reduces the postsynaptic inhibitory γ-aminobutyric acid-ergic tone and attenuates postovariectomy body weight gain in female guinea pigs through the administration of a selective Gq-mER ligand, STX.To determine the role of Gq-mER in regulating Tc, energy and bone homeostasis, ovariectomized female guinea pigs, implanted ip with temperature probes, were treated with STX or E2 for 7-8 wk. Tc was recorded for 4 wk,where as food intake and body weight were monitored daily. Bone density and fat accumulation were determined postmortem. Both E2 and STX significantly reduced Tc in the females compared with controls. STX, similar to E2, reduced food intake and fat accumulation and increased tibial bone density. Therefore, a Gq-mER-coupled signaling pathway appears to be involved in maintaining homeostatic functions and may constitute a novel therapeutic target for treatment of hypoestrogenic symptoms.
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U2 - 10.1210/en.2010-0573
DO - 10.1210/en.2010-0573
M3 - Article
C2 - 20685867
AN - SCOPUS:77957260252
SN - 0013-7227
VL - 151
SP - 4926
EP - 4937
JO - Endocrinology
JF - Endocrinology
IS - 10
ER -