Content of the neurotoxins cycasin (methylazoxymethanol β-D-glucoside) and BMAA (β-N-methylamino-L-alanine) in cycad flour prepared by guam chamorros

Glen E. Kisby, Mike Ellison, Peter Spencer

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

Exposure to cycad seed kernel is an etiologic factor for the western Pacific amyotrophic lateral sclerosis (ALS) and parkinsonism-dementia complex (PDC). Traditionally processed cycad flours (n = 17) obtained from Chamorro residents of Guam and the adjacent island of Rota at risk for neurodegenerative disease were extracted and analyzed by high-performance liquid chromatography for content of β-N-methylamino-L-alanine (BMAA) and methylazoxymethanol β-D-glucoside (cycasin). Cycasin (detection limit: picomole) was present in concentrations of 0.004 to 75.93 μg/g (mean, 12.45 ± 5.0 μg/g), and levels of BMAA (detection limit: subpicomole) ranged from 0.00 to 18.39 μg/g (mean, 5.44 ± 1.56 μg/g). On average, cycasin content was approximately 10 times higher than that of BMAA. The largest concentrations of cycasin were found in samples from villages with a high reported prevalence of ALS/PDC. Ingestion of cycad-derived food would result in estimated human exposure to milligram amounts of cycasin per day. The cytotoxic properties of cycasin merit consideration in relation to the etiology of western Pacific ALS/PDC.

Original languageEnglish (US)
Pages (from-to)1336-1340
Number of pages5
JournalNeurology
Volume42
Issue number7
StatePublished - 1992

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Cycasin
Guam
Glucosides
Neurotoxins
Flour
Alanine
Amyotrophic Lateral Sclerosis
Parkinsonian Disorders
Dementia
Limit of Detection
Islands
Neurodegenerative Diseases
methylazoxymethanol
Seeds
Eating
High Pressure Liquid Chromatography
Food

ASJC Scopus subject areas

  • Arts and Humanities (miscellaneous)
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Content of the neurotoxins cycasin (methylazoxymethanol β-D-glucoside) and BMAA (β-N-methylamino-L-alanine) in cycad flour prepared by guam chamorros. / Kisby, Glen E.; Ellison, Mike; Spencer, Peter.

In: Neurology, Vol. 42, No. 7, 1992, p. 1336-1340.

Research output: Contribution to journalArticle

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