Construction and characterization of combinatorial naive phage library of human scFvs

T. A. Batanova, A. B. Ulitin, V. V. Morozova, A. G. Laman, E. V. Zhirakovskaia, Viktoriya Dubrovskaya, F. A. Brovko, N. V. Tikunova

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A combinatorial phage display library of human single-chain antibody fragments (scFv) was constructed on the basis of variable domains of heavy (Vh) and light (VI) genes cloned from the lymphocytes of six healthy donors. The size of the library was 2? 10(8) independent clones. Single-chain antibodies against recombinant human TNF?, vaccinia virus and virus-like particles formed by core protein of hepatitis B virus were selected from the library. Unique scFv sequences were identified using the HaeIII fingerprinting. The specificity of the selected clones was proved by the Western-blot analysis.

Original languageEnglish (US)
Pages (from-to)35-41
Number of pages7
JournalMolekuliarnaia genetika, mikrobiologiia i virusologiia.
Issue number3
StatePublished - Sep 26 2006
Externally publishedYes

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Single-Chain Antibodies
Bacteriophages
Libraries
Clone Cells
Immunoglobulin Fragments
Vaccinia virus
Hepatitis B virus
Virion
Western Blotting
Lymphocytes
Light
Genes
IgA receptor

Cite this

Batanova, T. A., Ulitin, A. B., Morozova, V. V., Laman, A. G., Zhirakovskaia, E. V., Dubrovskaya, V., ... Tikunova, N. V. (2006). Construction and characterization of combinatorial naive phage library of human scFvs. Molekuliarnaia genetika, mikrobiologiia i virusologiia., (3), 35-41.

Construction and characterization of combinatorial naive phage library of human scFvs. / Batanova, T. A.; Ulitin, A. B.; Morozova, V. V.; Laman, A. G.; Zhirakovskaia, E. V.; Dubrovskaya, Viktoriya; Brovko, F. A.; Tikunova, N. V.

In: Molekuliarnaia genetika, mikrobiologiia i virusologiia., No. 3, 26.09.2006, p. 35-41.

Research output: Contribution to journalArticle

Batanova, TA, Ulitin, AB, Morozova, VV, Laman, AG, Zhirakovskaia, EV, Dubrovskaya, V, Brovko, FA & Tikunova, NV 2006, 'Construction and characterization of combinatorial naive phage library of human scFvs', Molekuliarnaia genetika, mikrobiologiia i virusologiia., no. 3, pp. 35-41.
Batanova, T. A. ; Ulitin, A. B. ; Morozova, V. V. ; Laman, A. G. ; Zhirakovskaia, E. V. ; Dubrovskaya, Viktoriya ; Brovko, F. A. ; Tikunova, N. V. / Construction and characterization of combinatorial naive phage library of human scFvs. In: Molekuliarnaia genetika, mikrobiologiia i virusologiia. 2006 ; No. 3. pp. 35-41.
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