Construction and analysis of recombinant DNAs containing a structural gene for rat prolactin

Earl J. Gubbins, Richard Maurer, James L. Hartley, John E. Donelson

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Poly A-containing RNA enriched in prolactin-coding sequences was isolated from female rat pituitaries after induction with diethylstilbesterol. Double stranded cDNA was synthesized from this RNA and inserted into plasmid pBR322 at the Pst I site via the poly(dG):poly(dC) tailing method. E. coli was transformed with this DNA and the recombinant plasmid in one of the transformants characterized in detail. About half of its 900 base pair cDNA insert was sequenced. The DNA sequence is consistent with most of the reported amino acid sequence of rat preprolactin. In addition, the recombinant plasmids in two of the other transformants appear to contain growth hormone coding sequences.

Original languageEnglish (US)
Pages (from-to)915-930
Number of pages16
JournalNucleic Acids Research
Volume6
Issue number3
DOIs
StatePublished - Mar 1979
Externally publishedYes

Fingerprint

Recombinant DNA
CDNA
RNA
Prolactin
Rats
Plasmids
Coding
Genes
Growth Hormone
Gene
DNA sequences
Hormones
Tailings
Amino Acid Sequence
Complementary DNA
DNA Sequence
Escherichia coli
Escherichia Coli
Amino acids
Proof by induction

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Construction and analysis of recombinant DNAs containing a structural gene for rat prolactin. / Gubbins, Earl J.; Maurer, Richard; Hartley, James L.; Donelson, John E.

In: Nucleic Acids Research, Vol. 6, No. 3, 03.1979, p. 915-930.

Research output: Contribution to journalArticle

Gubbins, Earl J. ; Maurer, Richard ; Hartley, James L. ; Donelson, John E. / Construction and analysis of recombinant DNAs containing a structural gene for rat prolactin. In: Nucleic Acids Research. 1979 ; Vol. 6, No. 3. pp. 915-930.
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