Construction and analysis of recombinant DNAs containing a structural gene for rat prolactin

Earl J. Gubbins; Richard A. Maurer; James L. Hartley; John E. Donelson

doi:10.1093/nar/6.3.915

Construction and analysis of recombinant DNAs containing a structural gene for rat prolactin

Earl J. Gubbins, Richard A. Maurer, James L. Hartley, John E. Donelson

Research output: Contribution to journal › Article › peer-review

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Abstract

Poly A-containing RNA enriched in prolactin-coding sequences was isolated from female rat pituitaries after induction with diethylstilbesterol. Double stranded cDNA was synthesized from this RNA and inserted into plasmid pBR322 at the Pst I site via the poly(dG):poly(dC) tailing method. E. coli was transformed with this DNA and the recombinant plasmid in one of the transformants characterized in detail. About half of its 900 base pair cDNA insert was sequenced. The DNA sequence is consistent with most of the reported amino acid sequence of rat preprolactin. In addition, the recombinant plasmids in two of the other transformants appear to contain growth hormone coding sequences.

Original language	English (US)
Pages (from-to)	915-930
Number of pages	16
Journal	Nucleic acids research
Volume	6
Issue number	3
DOIs	https://doi.org/10.1093/nar/6.3.915
State	Published - Mar 1979
Externally published	Yes

ASJC Scopus subject areas

Genetics

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title = "Construction and analysis of recombinant DNAs containing a structural gene for rat prolactin",

abstract = "Poly A-containing RNA enriched in prolactin-coding sequences was isolated from female rat pituitaries after induction with diethylstilbesterol. Double stranded cDNA was synthesized from this RNA and inserted into plasmid pBR322 at the Pst I site via the poly(dG):poly(dC) tailing method. E. coli was transformed with this DNA and the recombinant plasmid in one of the transformants characterized in detail. About half of its 900 base pair cDNA insert was sequenced. The DNA sequence is consistent with most of the reported amino acid sequence of rat preprolactin. In addition, the recombinant plasmids in two of the other transformants appear to contain growth hormone coding sequences.",

author = "Gubbins, {Earl J.} and Maurer, {Richard A.} and Hartley, {James L.} and Donelson, {John E.}",

note = "Funding Information: ACKNOWLEDGEMENTS This research was supported by N.I.H. grant AM 21803 to R.A.M. and N.S.F. grant PCM 76-13461 to J.E.D. E.J.G. was supported by predoctoral cell and molecular biology training grant GM-07228.",

year = "1979",

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doi = "10.1093/nar/6.3.915",

language = "English (US)",

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journal = "Nucleic acids research",

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T1 - Construction and analysis of recombinant DNAs containing a structural gene for rat prolactin

AU - Gubbins, Earl J.

AU - Maurer, Richard A.

AU - Hartley, James L.

AU - Donelson, John E.

N1 - Funding Information: ACKNOWLEDGEMENTS This research was supported by N.I.H. grant AM 21803 to R.A.M. and N.S.F. grant PCM 76-13461 to J.E.D. E.J.G. was supported by predoctoral cell and molecular biology training grant GM-07228.

PY - 1979/3

Y1 - 1979/3

N2 - Poly A-containing RNA enriched in prolactin-coding sequences was isolated from female rat pituitaries after induction with diethylstilbesterol. Double stranded cDNA was synthesized from this RNA and inserted into plasmid pBR322 at the Pst I site via the poly(dG):poly(dC) tailing method. E. coli was transformed with this DNA and the recombinant plasmid in one of the transformants characterized in detail. About half of its 900 base pair cDNA insert was sequenced. The DNA sequence is consistent with most of the reported amino acid sequence of rat preprolactin. In addition, the recombinant plasmids in two of the other transformants appear to contain growth hormone coding sequences.

AB - Poly A-containing RNA enriched in prolactin-coding sequences was isolated from female rat pituitaries after induction with diethylstilbesterol. Double stranded cDNA was synthesized from this RNA and inserted into plasmid pBR322 at the Pst I site via the poly(dG):poly(dC) tailing method. E. coli was transformed with this DNA and the recombinant plasmid in one of the transformants characterized in detail. About half of its 900 base pair cDNA insert was sequenced. The DNA sequence is consistent with most of the reported amino acid sequence of rat preprolactin. In addition, the recombinant plasmids in two of the other transformants appear to contain growth hormone coding sequences.

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Construction and analysis of recombinant DNAs containing a structural gene for rat prolactin

Abstract

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