Consistent patterns of change during the divergence of human immunodeficiency virus type 1 envelope from that of the inoculated virus in simian/human immunodeficiency virus-infected macaques

W. M. Blay, S. Gnanakaran, B. Foley, N. A. Dona-Rose, B. T. Korber, Nancy Haigwood

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Abstract

We have analyzed changes to proviral Env gp120 sequences and the development of neutralizing antibodies (NAbs) during 1 year of simian/human immunodeficiency virus SHIV-89.6P infection in 11 Macaca nemestrina macaques. Seven macaques had significant env divergence from that of the inoculum, and macaques with greater divergence had higher titers of homologous NAbs. Substitutions in sequons encoding potential N-linked glycosylation sites (PNGs) were among the first to be established, although overall the total number of sequons did not increase significantly. The majority (19 of 23) of PNGs present in the inoculum were conserved in the sequences from all macaques. Statistically significant variations in PNGs occurred in multiple macaques within constrained regions we term "hot spots," resulting in the selection of sequences more similar to the B consensus. These included additions on V1, the N-terminal side of V4, and the outer region of C2. Complex mutational patterns resulted in convergent PNG shifts in V2 and VS. Charge changes in Env V1V2, resulting in a net acidic charge, and a proline addition in V5 occurred in several macaques. Molecular modeling of the 89.6P sequence showed that the conserved glycans lie on the silent face of Env and that many are proximal to disulfide bonds, while PNG additions and shifts are proximal to the CD4 binding site. Nonsynonymous-to-synonymous substitution ratios suggest that these changes result from selective pressure. This longitudinal and cross-sectional study of mutations in human immunodeficiency virus (HIV) env in the SHIV background provides evidence that there are more constraints on the configuration of the glycan shield than were previously appreciated.

Original languageEnglish (US)
Pages (from-to)999-1014
Number of pages16
JournalJournal of Virology
Volume80
Issue number2
DOIs
StatePublished - Jan 2006
Externally publishedYes

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Simian Immunodeficiency Virus
Human immunodeficiency virus
Macaca
Human immunodeficiency virus 1
HIV-1
HIV
Viruses
viruses
conserved sequences
Conserved Sequence
Neutralizing Antibodies
neutralizing antibodies
Polysaccharides
inoculum
polysaccharides
Macaca nemestrina
disulfide bonds
glycosylation
Glycosylation
Proline

ASJC Scopus subject areas

  • Immunology

Cite this

Consistent patterns of change during the divergence of human immunodeficiency virus type 1 envelope from that of the inoculated virus in simian/human immunodeficiency virus-infected macaques. / Blay, W. M.; Gnanakaran, S.; Foley, B.; Dona-Rose, N. A.; Korber, B. T.; Haigwood, Nancy.

In: Journal of Virology, Vol. 80, No. 2, 01.2006, p. 999-1014.

Research output: Contribution to journalArticle

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