Connective tissue microfibrils

B. K. Maddox, Lynn Sakai, D. R. Keene, R. W. Glanville

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

Human amnion was solubilized using pepsin and the digest supernatant screened for fragments of fibrillin with a previously characterized monoclonal antibody [Sakai, L.Y., Keene, D.R., and Engvall, E. (1986) J. Cell Biol. 103, 2499-2509). One fragment (PF1), with an apparent molecular weight of 94,000, was isolated and characterized. Two other fragments, PF2 and PF3, were isolated and shown to be fragments of fibrillin by preparing a monospecific antisera to PF2 and a monoclonal antibody to PF3. Immunoelectron microscopy and immunoblotting showed that both antibodies were specific for fibrillin. Electron microscope pictures of rotary-shadowed PF1 and PF2 showed them to be short rod-shaped molecules while PF3 has a crab-like appearance and seems to be an aggregate of several fibrillin chain fragments. Amino-terminal amino acid sequencing of PF1 and PF2 gave single unique sequences. Each of the three antibodies used was specific for one fragment and peptide mapping of PF1 and PF2 showed that there was no significant amino acid sequence overlap. Aggregates of PF3 are described which provided insight into the assembly and macromolecular structure of fibrillin in microfibrils.

Original languageEnglish (US)
Pages (from-to)21381-21385
Number of pages5
JournalJournal of Biological Chemistry
Volume264
Issue number35
StatePublished - Dec 1 1989
Externally publishedYes

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Microfibrils
Connective Tissue
Monoclonal Antibodies
Tissue
Amino Acids
Peptide Fragments
Antibodies
Pepsin A
Immune Sera
Microscopic examination
Electron microscopes
Molecular weight
Molecules
Amnion
Peptide Mapping
Immunoelectron Microscopy
Protein Sequence Analysis
Immunoblotting
Amino Acid Sequence
Molecular Weight

ASJC Scopus subject areas

  • Biochemistry

Cite this

Maddox, B. K., Sakai, L., Keene, D. R., & Glanville, R. W. (1989). Connective tissue microfibrils. Journal of Biological Chemistry, 264(35), 21381-21385.

Connective tissue microfibrils. / Maddox, B. K.; Sakai, Lynn; Keene, D. R.; Glanville, R. W.

In: Journal of Biological Chemistry, Vol. 264, No. 35, 01.12.1989, p. 21381-21385.

Research output: Contribution to journalArticle

Maddox, BK, Sakai, L, Keene, DR & Glanville, RW 1989, 'Connective tissue microfibrils', Journal of Biological Chemistry, vol. 264, no. 35, pp. 21381-21385.
Maddox BK, Sakai L, Keene DR, Glanville RW. Connective tissue microfibrils. Journal of Biological Chemistry. 1989 Dec 1;264(35):21381-21385.
Maddox, B. K. ; Sakai, Lynn ; Keene, D. R. ; Glanville, R. W. / Connective tissue microfibrils. In: Journal of Biological Chemistry. 1989 ; Vol. 264, No. 35. pp. 21381-21385.
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