Confocal microscopy measures tissue optical properties

Steven Jacques, Daniel S. Gareau

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

The signal from a confocal measurement as the focal volume is scanned down into a tissue yields an exponential decay versus depth z focus, signal = ρ exp(-μ z focus), where p [dimensionless] is the local reflectivity and μ [1/cm] is an attenuation coefficient. A simple theory for how ρ and |a depend on the optical properties of scattering (μ s) and anisotropy (g) is presented. Experimental measurements on 5 tissue types from mice (white and gray matter of brain, skin, liver, muscle) as well as 0.1-μm-dia. polystyrene microspheres are presented. The tissues have similar μ s values (about 500 [1/cm]) but variable g values (0.8-0.99). Anisotropy appears to be the primary mechanism of contrast for confocal measurements such as reflectance-mode confocal laser scanning microscopy (rCLSM) and optical coherence tomography (OCT). While fluorescence imaging depends on fluorophores, and absorption imaging depends on chromophores, the results of this study suggest that contrast of confocal imaging of biological tissues depends primarily on anisotropy.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6163
DOIs
StatePublished - 2006
EventSaratov Fall Meeting 2005: Optical Technologies in Biophysics and Medicine VII - Saratov, Russian Federation
Duration: Sep 27 2005Sep 30 2005

Other

OtherSaratov Fall Meeting 2005: Optical Technologies in Biophysics and Medicine VII
CountryRussian Federation
CitySaratov
Period9/27/059/30/05

Fingerprint

Confocal microscopy
Optical properties
Tissue
Anisotropy
Imaging techniques
Fluorophores
Optical tomography
Chromophores
Laser modes
Microspheres
Liver
Muscle
Polystyrenes
Brain
Skin
Microscopic examination
Fluorescence
Scattering
Scanning
Lasers

Keywords

  • Anisotropy
  • Confocal microscopy
  • Optical coherence tomography
  • Optical imaging
  • Optical properties
  • Tissue optics

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Jacques, S., & Gareau, D. S. (2006). Confocal microscopy measures tissue optical properties. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 6163). [61630X] https://doi.org/10.1117/12.697298

Confocal microscopy measures tissue optical properties. / Jacques, Steven; Gareau, Daniel S.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6163 2006. 61630X.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Jacques, S & Gareau, DS 2006, Confocal microscopy measures tissue optical properties. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 6163, 61630X, Saratov Fall Meeting 2005: Optical Technologies in Biophysics and Medicine VII, Saratov, Russian Federation, 9/27/05. https://doi.org/10.1117/12.697298
Jacques S, Gareau DS. Confocal microscopy measures tissue optical properties. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6163. 2006. 61630X https://doi.org/10.1117/12.697298
Jacques, Steven ; Gareau, Daniel S. / Confocal microscopy measures tissue optical properties. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6163 2006.
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