Conflicting indicators of biotin status from a cross-sectional study of normal pregnancy

Donald M. Mock, Diane Stadler

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Objective: To assess biotin nutritional status during normal human gestation. Methods: Urine samples were obtained in a cross-sectional design from 16 women in early pregnancy (17 ± 1 weeks, mean ± 1 SD) and from 13 women in late pregnancy (36 ± 1 weeks). The urinary excretion of biotin, two metabolites bisnorbiotin (BNB) and biotin sulfoxide (BSO), and the organic acid 3-hydroxyisovaleric acid (3-HIA) were measured by HPLC/avidin-binding assay and GC/MS, respectively. Excretion rates were expressed as concentration ratios to urinary creatinine. Results: In both early and late pregnancy, 3-HIA excretion was increased compared to controls (p <0.0001), suggesting decreased activity of a biotin-dependent enzyme caused by tissue biotin depletion. In early pregnancy, urinary excretion of biotin was normal; in late pregnancy, excretion was increased (p <0.0002), suggesting biotin status was not decreased. In late pregnancy, urinary excretion of BNB and BSO were increased (p <0.009). Conclusion: The apparent conflict in the indices of biotin status is not explained by this study but could be resolved by two alternate explanations: 1) Pregnancy caused an impairment of renal reclamation of biotin, BNB, and BSO leading to a paradoxical increase in biotin excretion. 2) Pregnancy caused metabolic or renal effects that increased 3-HIA excretion nonspecifically; hence, the increased 3-HIA excretion did not reflect biotin deficiency. We speculate that some of the women studied were marginally biotin deficient and that renal wasting and accelerated breakdown of biotin contributed to the deficiency.

Original languageEnglish (US)
Pages (from-to)252-257
Number of pages6
JournalJournal of the American College of Nutrition
Volume16
Issue number3
StatePublished - Jun 1997
Externally publishedYes

Fingerprint

biotin
Biotin
cross-sectional studies
Cross-Sectional Studies
pregnancy
Pregnancy
excretion
Kidney
acids
kidneys
Avidin
Nutritional Status
avidin
Creatinine
High Pressure Liquid Chromatography
Urine
creatinine
organic acids and salts
nutritional status
Acids

Keywords

  • 3-hydroxyisovaleric acid
  • avitaminosis
  • biotin
  • human
  • pregnancy

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

Cite this

Conflicting indicators of biotin status from a cross-sectional study of normal pregnancy. / Mock, Donald M.; Stadler, Diane.

In: Journal of the American College of Nutrition, Vol. 16, No. 3, 06.1997, p. 252-257.

Research output: Contribution to journalArticle

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