Conductance properties of GABA-activated chloride currents recorded from cultured hippocampal neurons

C. N. Allen; E. X. Albuquerque

doi:10.1016/S0006-8993(87)80039-2

Conductance properties of GABA-activated chloride currents recorded from cultured hippocampal neurons

C. N. Allen, E. X. Albuquerque

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

The conductance characteristics of γ-aminobutyric acid-activated single channel currents from cultured hippocampal neurons were examined using patch clamp techniques. GABA-activated currents had amplitudes which were linearly correlated to the membrane potentials over a range of -80 to +70 mV and an open time and burst time of 2.2 and 4.3 ms, respectively. The conductance of the γ-aminobutyric acid-activated channels was 19 pS. These data demonstrate that cultured hippocampal neurons have channel conductances which have characteristics different from those of adult neurons.

Original language	English (US)
Pages (from-to)	159-163
Number of pages	5
Journal	Brain research
Volume	410
Issue number	1
DOIs	https://doi.org/10.1016/S0006-8993(87)80039-2
State	Published - Apr 28 1987
Externally published	Yes

Keywords

Amino acid
Cultured hippocampal pyramidal cell
Patch clamp recording
Single channel conductance
Single chloride channel
γ-Aminobutyric acid

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/S0006-8993(87)80039-2

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title = "Conductance properties of GABA-activated chloride currents recorded from cultured hippocampal neurons",

abstract = "The conductance characteristics of γ-aminobutyric acid-activated single channel currents from cultured hippocampal neurons were examined using patch clamp techniques. GABA-activated currents had amplitudes which were linearly correlated to the membrane potentials over a range of -80 to +70 mV and an open time and burst time of 2.2 and 4.3 ms, respectively. The conductance of the γ-aminobutyric acid-activated channels was 19 pS. These data demonstrate that cultured hippocampal neurons have channel conductances which have characteristics different from those of adult neurons.",

keywords = "Amino acid, Cultured hippocampal pyramidal cell, Patch clamp recording, Single channel conductance, Single chloride channel, γ-Aminobutyric acid",

author = "Allen, {C. N.} and Albuquerque, {E. X.}",

note = "Funding Information: We are indebted to Dr. Neville Brookes for the use of the cell culture facilities and many helpful suggestions. We thank Mrs. Yvonne Logan for skillful technical assistance and Ms. Mabel Zelle for expert computer programming. This project was supported by U.S. Army Medical Research Development Command Contract DAMD-17-84-C-4219.",

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doi = "10.1016/S0006-8993(87)80039-2",

language = "English (US)",

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pages = "159--163",

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T1 - Conductance properties of GABA-activated chloride currents recorded from cultured hippocampal neurons

AU - Allen, C. N.

AU - Albuquerque, E. X.

N1 - Funding Information: We are indebted to Dr. Neville Brookes for the use of the cell culture facilities and many helpful suggestions. We thank Mrs. Yvonne Logan for skillful technical assistance and Ms. Mabel Zelle for expert computer programming. This project was supported by U.S. Army Medical Research Development Command Contract DAMD-17-84-C-4219.

PY - 1987/4/28

Y1 - 1987/4/28

N2 - The conductance characteristics of γ-aminobutyric acid-activated single channel currents from cultured hippocampal neurons were examined using patch clamp techniques. GABA-activated currents had amplitudes which were linearly correlated to the membrane potentials over a range of -80 to +70 mV and an open time and burst time of 2.2 and 4.3 ms, respectively. The conductance of the γ-aminobutyric acid-activated channels was 19 pS. These data demonstrate that cultured hippocampal neurons have channel conductances which have characteristics different from those of adult neurons.

AB - The conductance characteristics of γ-aminobutyric acid-activated single channel currents from cultured hippocampal neurons were examined using patch clamp techniques. GABA-activated currents had amplitudes which were linearly correlated to the membrane potentials over a range of -80 to +70 mV and an open time and burst time of 2.2 and 4.3 ms, respectively. The conductance of the γ-aminobutyric acid-activated channels was 19 pS. These data demonstrate that cultured hippocampal neurons have channel conductances which have characteristics different from those of adult neurons.

KW - Amino acid

KW - Cultured hippocampal pyramidal cell

KW - Patch clamp recording

KW - Single channel conductance

KW - Single chloride channel

KW - γ-Aminobutyric acid

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Conductance properties of GABA-activated chloride currents recorded from cultured hippocampal neurons

Abstract

Keywords

ASJC Scopus subject areas

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