Conditional infectivity of a human immunodeficiency virus matrix domain deletion mutant

Chin Tien Wang, Yaqiang Zhang, Jason McDermott, Eric Barklis

Research output: Contribution to journalArticle

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Abstract

We constructed a human immunodeficiency virus (HIV) matrix (MA) deletion mutant by deletion of about 80% of the HIV type 1 Gag MA domain but retaining myristylation and proteolytic processing signals. The effects of this deletion matrix (dl.MA) mutant on HIV particle assembly, processing, and infectivity were analyzed. Surprisingly, the dl.MA mutant still could assemble and process virus particles, had a wild-type (wt) retrovirus particle density, and possessed wt reverse transcriptase activity. RNase protection experiments showed that dl.MA mutant particles preferentially packaged viral genomic RNA. When both mutant and wt particles were pseudotyped with an amphotropic murine leukemia virus envelope protein, mutant infectivity was about 10% of wt level. In contrast, infectivity of the dl.MA mutant was 1,000-fold less than that of wild-type when mutant and wt particles were pseudotyped with the HIV envelope protein. Protein analyses of pseudotyped virions indicated that there were no major differences between mutant and wt viruses in the efficiency of amphotropic murine leukemia virus envelope protein incorporation. In contrast, there was a reduction in the amount of mutant particle-associated HIV envelope protein gp120. Our results suggest that an intact HIV matrix domain is not absolutely required for reverse transcription, nuclear localization, or integration but is necessary for appropriate HIV envelope protein function.

Original languageEnglish (US)
Pages (from-to)7067-7076
Number of pages10
JournalJournal of Virology
Volume67
Issue number12
StatePublished - Dec 1993

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Human Immunodeficiency Virus env Gene Products
Human immunodeficiency virus
Virion
Viral Envelope Proteins
Murine Leukemia Viruses
pathogenicity
HIV
mutants
Satellite Viruses
Virus Assembly
RNA-Directed DNA Polymerase
Viral RNA
Retroviridae
Ribonucleases
Reverse Transcription
virion
Murine leukemia virus
HIV-1
proteins
Viruses

ASJC Scopus subject areas

  • Immunology

Cite this

Conditional infectivity of a human immunodeficiency virus matrix domain deletion mutant. / Wang, Chin Tien; Zhang, Yaqiang; McDermott, Jason; Barklis, Eric.

In: Journal of Virology, Vol. 67, No. 12, 12.1993, p. 7067-7076.

Research output: Contribution to journalArticle

Wang, Chin Tien ; Zhang, Yaqiang ; McDermott, Jason ; Barklis, Eric. / Conditional infectivity of a human immunodeficiency virus matrix domain deletion mutant. In: Journal of Virology. 1993 ; Vol. 67, No. 12. pp. 7067-7076.
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