Comprehensive proteomic analysis of human cervical-vaginal fluid

Surendra Dasari, Leonardo Pereira, Ashok P. Reddy, John Edward A Michaels, Xinfang Lu, Thomas Jacob, Archana Thomas, Matthew Rodland, Charles Roberts, Michael G. Gravett, Srinivasa R. Nagalla

Research output: Contribution to journalArticle

95 Citations (Scopus)

Abstract

Cervical-vaginal fluid (CVF) is a potential rich source of biomarkers for enhancing our understanding of human parturition and pathologic conditions affecting pregnancy. In this study, we performed a comprehensive survey of the CVF proteome in pregnancy utilizing multidimensional liquid chromatography (2D-LC) coupled with mass spectrometry and gel-electrophoresis-based protein separation and identification. In total, 150 unique proteins were identified using multiple protein identification algorithms. Metabolism (32%) and immune response-related (22%) proteins are the major functional categories represented in the CVF proteome. A comparison of the CVF, serum, and amniotic fluid proteomes showed that 77 proteins are unique to CVF, while 56 and 17 CVF proteins also occur in serum and amniotic fluid, respectively. This data set provides a foundation for evaluation of these proteins as potential CVF biomarkers for noninvasive diagnosis of pregnancy-related disorders.

Original languageEnglish (US)
Pages (from-to)1258-1268
Number of pages11
JournalJournal of Proteome Research
Volume6
Issue number4
DOIs
StatePublished - Apr 2007

Fingerprint

Proteomics
Fluids
Proteome
Proteins
Amniotic Fluid
Pregnancy
Biomarkers
Serum
Liquid Chromatography
Electrophoresis
Liquid chromatography
Mass Spectrometry
Metabolism
Gels
Parturition
Mass spectrometry

Keywords

  • Pregnancy
  • Prematurity
  • Proteome
  • Vaginal fluid

ASJC Scopus subject areas

  • Genetics
  • Biotechnology
  • Biochemistry

Cite this

Dasari, S., Pereira, L., Reddy, A. P., Michaels, J. E. A., Lu, X., Jacob, T., ... Nagalla, S. R. (2007). Comprehensive proteomic analysis of human cervical-vaginal fluid. Journal of Proteome Research, 6(4), 1258-1268. https://doi.org/10.1021/pr0605419

Comprehensive proteomic analysis of human cervical-vaginal fluid. / Dasari, Surendra; Pereira, Leonardo; Reddy, Ashok P.; Michaels, John Edward A; Lu, Xinfang; Jacob, Thomas; Thomas, Archana; Rodland, Matthew; Roberts, Charles; Gravett, Michael G.; Nagalla, Srinivasa R.

In: Journal of Proteome Research, Vol. 6, No. 4, 04.2007, p. 1258-1268.

Research output: Contribution to journalArticle

Dasari, S, Pereira, L, Reddy, AP, Michaels, JEA, Lu, X, Jacob, T, Thomas, A, Rodland, M, Roberts, C, Gravett, MG & Nagalla, SR 2007, 'Comprehensive proteomic analysis of human cervical-vaginal fluid', Journal of Proteome Research, vol. 6, no. 4, pp. 1258-1268. https://doi.org/10.1021/pr0605419
Dasari, Surendra ; Pereira, Leonardo ; Reddy, Ashok P. ; Michaels, John Edward A ; Lu, Xinfang ; Jacob, Thomas ; Thomas, Archana ; Rodland, Matthew ; Roberts, Charles ; Gravett, Michael G. ; Nagalla, Srinivasa R. / Comprehensive proteomic analysis of human cervical-vaginal fluid. In: Journal of Proteome Research. 2007 ; Vol. 6, No. 4. pp. 1258-1268.
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