Comprehensive identification of cell cycle-regulated genes of the yeast Saccharomyces cerevisiae by microarray hybridization

Paul T. Spellman, Gavin Sherlock, Michael Q. Zhang, Vishwanath R. Iyer, Kirk Anders, Michael B. Eisen, Patrick O. Brown, David Botstein, Bruce Futcher

Research output: Contribution to journalArticlepeer-review

3748 Scopus citations

Abstract

We sought to create a comprehensive catalog of yeast genes whose transcript levels vary periodically within the cell cycle. To this end, we used DNA microarrays and samples from yeast cultures synchronized by three independent methods: α factor arrest, elutriation, and arrest of a cdc15 temperature-sensitive mutant. Using periodicity and correlation algorithms, we identified 800 genes that meet an objective minimum criterion for cell cycle regulation. In separate experiments, designed to examine the effects of inducing either the G1 cyclin Cln3p or the B-type cyclin Clb2p, we found that the mRNA levels of more than half of these 800 genes respond to one or both of these cyclins. Furthermore, we analyzed our set of cell cycle-regulated genes for known and new promoter elements and show that several known elements (or variations thereof) contain information predictive of cell cycle regulation. A full description and complete data sets are available at http://cellcycle-www.stanford.edu.

Original languageEnglish (US)
Pages (from-to)3273-3297
Number of pages25
JournalMolecular biology of the cell
Volume9
Issue number12
DOIs
StatePublished - Dec 1998
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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