Comprehensive human cell-type methylation atlas reveals origins of circulating cell-free DNA in health and disease

Joshua Moss, Judith Magenheim, Daniel Neiman, Hai Zemmour, Netanel Loyfer, Amit Korach, Yaacov Samet, Myriam Maoz, Henrik Druid, Peter Arner, Keng Yeh Fu, Endre Kiss, Kirsty L. Spalding, Giora Landesberg, Aviad Zick, Albert Grinshpun, A. M.James Shapiro, Markus Grompe, Avigail Dreazan Wittenberg, Benjamin GlaserRuth Shemer, Tommy Kaplan, Yuval Dor

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Methylation patterns of circulating cell-free DNA (cfDNA) contain rich information about recent cell death events in the body. Here, we present an approach for unbiased determination of the tissue origins of cfDNA, using a reference methylation atlas of 25 human tissues and cell types. The method is validated using in silico simulations as well as in vitro mixes of DNA from different tissue sources at known proportions. We show that plasma cfDNA of healthy donors originates from white blood cells (55%), erythrocyte progenitors (30%), vascular endothelial cells (10%) and hepatocytes (1%). Deconvolution of cfDNA from patients reveals tissue contributions that agree with clinical findings in sepsis, islet transplantation, cancer of the colon, lung, breast and prostate, and cancer of unknown primary. We propose a procedure which can be easily adapted to study the cellular contributors to cfDNA in many settings, opening a broad window into healthy and pathologic human tissue dynamics.

Original languageEnglish (US)
Article number5068
JournalNature Communications
Volume9
Issue number1
DOIs
StatePublished - Dec 1 2018

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methylation
Methylation
Atlases
health
deoxyribonucleic acid
Cells
Health
Tissue
DNA
cells
Prostatic Neoplasms
cancer
transplantation
Islets of Langerhans Transplantation
leukocytes
Endothelial cells
erythrocytes
Deconvolution
Cell death
Plasma Cells

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Comprehensive human cell-type methylation atlas reveals origins of circulating cell-free DNA in health and disease. / Moss, Joshua; Magenheim, Judith; Neiman, Daniel; Zemmour, Hai; Loyfer, Netanel; Korach, Amit; Samet, Yaacov; Maoz, Myriam; Druid, Henrik; Arner, Peter; Fu, Keng Yeh; Kiss, Endre; Spalding, Kirsty L.; Landesberg, Giora; Zick, Aviad; Grinshpun, Albert; Shapiro, A. M.James; Grompe, Markus; Wittenberg, Avigail Dreazan; Glaser, Benjamin; Shemer, Ruth; Kaplan, Tommy; Dor, Yuval.

In: Nature Communications, Vol. 9, No. 1, 5068, 01.12.2018.

Research output: Contribution to journalArticle

Moss, J, Magenheim, J, Neiman, D, Zemmour, H, Loyfer, N, Korach, A, Samet, Y, Maoz, M, Druid, H, Arner, P, Fu, KY, Kiss, E, Spalding, KL, Landesberg, G, Zick, A, Grinshpun, A, Shapiro, AMJ, Grompe, M, Wittenberg, AD, Glaser, B, Shemer, R, Kaplan, T & Dor, Y 2018, 'Comprehensive human cell-type methylation atlas reveals origins of circulating cell-free DNA in health and disease', Nature Communications, vol. 9, no. 1, 5068. https://doi.org/10.1038/s41467-018-07466-6
Moss, Joshua ; Magenheim, Judith ; Neiman, Daniel ; Zemmour, Hai ; Loyfer, Netanel ; Korach, Amit ; Samet, Yaacov ; Maoz, Myriam ; Druid, Henrik ; Arner, Peter ; Fu, Keng Yeh ; Kiss, Endre ; Spalding, Kirsty L. ; Landesberg, Giora ; Zick, Aviad ; Grinshpun, Albert ; Shapiro, A. M.James ; Grompe, Markus ; Wittenberg, Avigail Dreazan ; Glaser, Benjamin ; Shemer, Ruth ; Kaplan, Tommy ; Dor, Yuval. / Comprehensive human cell-type methylation atlas reveals origins of circulating cell-free DNA in health and disease. In: Nature Communications. 2018 ; Vol. 9, No. 1.
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