TY - JOUR
T1 - Comprehensive analysis of varicella-zoster virus proteins using a new monoclonal antibody collection
AU - Roviš, Tihana Lenac
AU - Bailer, Susanne M.
AU - Pothineni, Venkata R.
AU - Ouwendijk, Werner J.D.
AU - Šimić, Hrvoje
AU - Babić, Marina
AU - Miklić, Karmela
AU - Malić, Suzana
AU - Verweij, Marieke C.
AU - Baiker, Armin
AU - Gonzalez, Orland
AU - Brunn, Albrecht von
AU - Zimmer, Ralf
AU - Früh, Klaus
AU - Verjans, Georges M.G.M.
AU - Jonjić, Stipan
AU - Haasb, Jürgeni
PY - 2013/6
Y1 - 2013/6
N2 - Varicella-zoster virus (VZV) is the etiological agent of chickenpox and shingles. Due to the virus's restricted host and cell typetropism and the lack of tools for VZV proteomics, it is one of the least-characterized human herpesviruses. We generated 251monoclonal antibodies (MAbs) against 59 of the 71 (83%) currently known unique VZV proteins to characterize VZV proteinexpression in vitro and in situ. Using this new set of MAbs, 44 viral proteins were detected by Western blotting (WB) and indirectimmunofluorescence (IF); 13 were detected by WB only, and 2 were detected by IF only. A large proportion of viral proteinswas analyzed for the first time in the context of virus infection. Our study revealed the subcellular localization of 46 proteins, 14of which were analyzed in detail by confocal microscopy. Seven viral proteins were analyzed in time course experiments andshowed a cascade-like temporal gene expression pattern similar to those of other herpesviruses. Furthermore, selected MAbstested positive on human skin lesions by using immunohistochemistry, demonstrating the wide applicability of the MAb collection.Finally, a significant portion of the VZV-specific antibodies reacted with orthologs of simian varicella virus (SVV), thusenabling the systematic analysis of varicella in a nonhuman primate model system. In summary, this study provides insight intothe potential function of numerous VZV proteins and novel tools to systematically study VZV and SVV pathogenesis.
AB - Varicella-zoster virus (VZV) is the etiological agent of chickenpox and shingles. Due to the virus's restricted host and cell typetropism and the lack of tools for VZV proteomics, it is one of the least-characterized human herpesviruses. We generated 251monoclonal antibodies (MAbs) against 59 of the 71 (83%) currently known unique VZV proteins to characterize VZV proteinexpression in vitro and in situ. Using this new set of MAbs, 44 viral proteins were detected by Western blotting (WB) and indirectimmunofluorescence (IF); 13 were detected by WB only, and 2 were detected by IF only. A large proportion of viral proteinswas analyzed for the first time in the context of virus infection. Our study revealed the subcellular localization of 46 proteins, 14of which were analyzed in detail by confocal microscopy. Seven viral proteins were analyzed in time course experiments andshowed a cascade-like temporal gene expression pattern similar to those of other herpesviruses. Furthermore, selected MAbstested positive on human skin lesions by using immunohistochemistry, demonstrating the wide applicability of the MAb collection.Finally, a significant portion of the VZV-specific antibodies reacted with orthologs of simian varicella virus (SVV), thusenabling the systematic analysis of varicella in a nonhuman primate model system. In summary, this study provides insight intothe potential function of numerous VZV proteins and novel tools to systematically study VZV and SVV pathogenesis.
UR - http://www.scopus.com/inward/record.url?scp=84878552793&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84878552793&partnerID=8YFLogxK
U2 - 10.1128/JVI.00407-13
DO - 10.1128/JVI.00407-13
M3 - Article
C2 - 23596286
AN - SCOPUS:84878552793
SN - 0022-538X
VL - 87
SP - 6943
EP - 6954
JO - Journal of virology
JF - Journal of virology
IS - 12
ER -