Complex interactions with direct repeats of a mitogen-responsive VL30 enhancer

David Pribnow, Steven Li Ye Chen, Yuan Zhang, Bruce E. Magun

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The RVL3 VL30 enhancer is an LTR-derived triple direct repeat of 35 base pairs that mediates gene induction in response to several different intracellular signaling pathways. Using mobility shift assays, methylation interference and DNase I footprinting, we have investigated the physical interactions between the RVL-3 enhancer and components of nuclear extracts from Rat-1 cells. Each enhancer repeat unit contains a single binding site. Our studies suggest that binding to the double or triple repeat enhancer is cooperative, involving simultaneous occupation of two sites, with a preference for adjacent sites. Binding cooperativity would have implications for the mechanism of gene activation directed from the native VL30 enhancer.

Original languageEnglish (US)
Pages (from-to)55-65
Number of pages11
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1307
Issue number1
DOIs
StatePublished - Jun 3 1996

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Nucleic Acid Repetitive Sequences
Deoxyribonuclease I
Electrophoretic Mobility Shift Assay
Mitogens
Occupations
Base Pairing
Methylation
Transcriptional Activation
Genes
Binding Sites
Rats
Assays
Chemical activation

Keywords

  • Cooperative binding
  • CREB/JUN
  • Enhancer
  • VL30

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Structural Biology
  • Biophysics

Cite this

Complex interactions with direct repeats of a mitogen-responsive VL30 enhancer. / Pribnow, David; Chen, Steven Li Ye; Zhang, Yuan; Magun, Bruce E.

In: Biochimica et Biophysica Acta - Gene Structure and Expression, Vol. 1307, No. 1, 03.06.1996, p. 55-65.

Research output: Contribution to journalArticle

Pribnow, David ; Chen, Steven Li Ye ; Zhang, Yuan ; Magun, Bruce E. / Complex interactions with direct repeats of a mitogen-responsive VL30 enhancer. In: Biochimica et Biophysica Acta - Gene Structure and Expression. 1996 ; Vol. 1307, No. 1. pp. 55-65.
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