Complex interactions with direct repeats of a mitogen-responsive VL30 enhancer

David Pribnow; Steven Li Ye Chen; Yuan Zhang; Bruce E. Magun

doi:10.1016/0167-4781(95)00218-9

Complex interactions with direct repeats of a mitogen-responsive VL30 enhancer

David Pribnow, Steven Li Ye Chen, Yuan Zhang, Bruce E. Magun

Cell Developmental and Cancer Biology

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

The RVL3 VL30 enhancer is an LTR-derived triple direct repeat of 35 base pairs that mediates gene induction in response to several different intracellular signaling pathways. Using mobility shift assays, methylation interference and DNase I footprinting, we have investigated the physical interactions between the RVL-3 enhancer and components of nuclear extracts from Rat-1 cells. Each enhancer repeat unit contains a single binding site. Our studies suggest that binding to the double or triple repeat enhancer is cooperative, involving simultaneous occupation of two sites, with a preference for adjacent sites. Binding cooperativity would have implications for the mechanism of gene activation directed from the native VL30 enhancer.

Original language	English (US)
Pages (from-to)	55-65
Number of pages	11
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1307
Issue number	1
DOIs	https://doi.org/10.1016/0167-4781(95)00218-9
State	Published - Jun 3 1996

Keywords

CREB/JUN
Cooperative binding
Enhancer
VL30

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

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10.1016/0167-4781(95)00218-9

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title = "Complex interactions with direct repeats of a mitogen-responsive VL30 enhancer",

abstract = "The RVL3 VL30 enhancer is an LTR-derived triple direct repeat of 35 base pairs that mediates gene induction in response to several different intracellular signaling pathways. Using mobility shift assays, methylation interference and DNase I footprinting, we have investigated the physical interactions between the RVL-3 enhancer and components of nuclear extracts from Rat-1 cells. Each enhancer repeat unit contains a single binding site. Our studies suggest that binding to the double or triple repeat enhancer is cooperative, involving simultaneous occupation of two sites, with a preference for adjacent sites. Binding cooperativity would have implications for the mechanism of gene activation directed from the native VL30 enhancer.",

keywords = "CREB/JUN, Cooperative binding, Enhancer, VL30",

author = "David Pribnow and Chen, {Steven Li Ye} and Yuan Zhang and Magun, {Bruce E.}",

note = "Funding Information: The authors appreciate the competent technical assistance of Jean Pearson, Thanh-Haoi Dinh, Kirsten Taylor and Sean Yoder. They thank Karin Rodland tbr sharing unpublished results and thank Richard Maurer tbr helpful criticism of the manuscript. This work was generously supported by grants DK44239 and CA39360 from the National Institutes ot' Health,.",

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doi = "10.1016/0167-4781(95)00218-9",

language = "English (US)",

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T1 - Complex interactions with direct repeats of a mitogen-responsive VL30 enhancer

AU - Pribnow, David

AU - Chen, Steven Li Ye

AU - Zhang, Yuan

AU - Magun, Bruce E.

N1 - Funding Information: The authors appreciate the competent technical assistance of Jean Pearson, Thanh-Haoi Dinh, Kirsten Taylor and Sean Yoder. They thank Karin Rodland tbr sharing unpublished results and thank Richard Maurer tbr helpful criticism of the manuscript. This work was generously supported by grants DK44239 and CA39360 from the National Institutes ot' Health,.

PY - 1996/6/3

Y1 - 1996/6/3

N2 - The RVL3 VL30 enhancer is an LTR-derived triple direct repeat of 35 base pairs that mediates gene induction in response to several different intracellular signaling pathways. Using mobility shift assays, methylation interference and DNase I footprinting, we have investigated the physical interactions between the RVL-3 enhancer and components of nuclear extracts from Rat-1 cells. Each enhancer repeat unit contains a single binding site. Our studies suggest that binding to the double or triple repeat enhancer is cooperative, involving simultaneous occupation of two sites, with a preference for adjacent sites. Binding cooperativity would have implications for the mechanism of gene activation directed from the native VL30 enhancer.

AB - The RVL3 VL30 enhancer is an LTR-derived triple direct repeat of 35 base pairs that mediates gene induction in response to several different intracellular signaling pathways. Using mobility shift assays, methylation interference and DNase I footprinting, we have investigated the physical interactions between the RVL-3 enhancer and components of nuclear extracts from Rat-1 cells. Each enhancer repeat unit contains a single binding site. Our studies suggest that binding to the double or triple repeat enhancer is cooperative, involving simultaneous occupation of two sites, with a preference for adjacent sites. Binding cooperativity would have implications for the mechanism of gene activation directed from the native VL30 enhancer.

KW - CREB/JUN

KW - Cooperative binding

KW - Enhancer

KW - VL30

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Complex interactions with direct repeats of a mitogen-responsive VL30 enhancer

Abstract

Keywords

ASJC Scopus subject areas

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