Complex interactions with direct repeats of a mitogen-responsive VL30 enhancer

David Pribnow, Steven Li Ye Chen, Yuan Zhang, Bruce E. Magun

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

The RVL3 VL30 enhancer is an LTR-derived triple direct repeat of 35 base pairs that mediates gene induction in response to several different intracellular signaling pathways. Using mobility shift assays, methylation interference and DNase I footprinting, we have investigated the physical interactions between the RVL-3 enhancer and components of nuclear extracts from Rat-1 cells. Each enhancer repeat unit contains a single binding site. Our studies suggest that binding to the double or triple repeat enhancer is cooperative, involving simultaneous occupation of two sites, with a preference for adjacent sites. Binding cooperativity would have implications for the mechanism of gene activation directed from the native VL30 enhancer.

Original languageEnglish (US)
Pages (from-to)55-65
Number of pages11
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1307
Issue number1
DOIs
StatePublished - Jun 3 1996

Keywords

  • CREB/JUN
  • Cooperative binding
  • Enhancer
  • VL30

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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