Complex interactions with direct repeats of a mitogen-responsive VL30 enhancer

David Pribnow, Steven Li Ye Chen, Yuan Zhang, Bruce E. Magun

    Research output: Contribution to journalArticlepeer-review

    1 Scopus citations

    Abstract

    The RVL3 VL30 enhancer is an LTR-derived triple direct repeat of 35 base pairs that mediates gene induction in response to several different intracellular signaling pathways. Using mobility shift assays, methylation interference and DNase I footprinting, we have investigated the physical interactions between the RVL-3 enhancer and components of nuclear extracts from Rat-1 cells. Each enhancer repeat unit contains a single binding site. Our studies suggest that binding to the double or triple repeat enhancer is cooperative, involving simultaneous occupation of two sites, with a preference for adjacent sites. Binding cooperativity would have implications for the mechanism of gene activation directed from the native VL30 enhancer.

    Original languageEnglish (US)
    Pages (from-to)55-65
    Number of pages11
    JournalBiochimica et Biophysica Acta - Gene Structure and Expression
    Volume1307
    Issue number1
    DOIs
    StatePublished - Jun 3 1996

    Keywords

    • CREB/JUN
    • Cooperative binding
    • Enhancer
    • VL30

    ASJC Scopus subject areas

    • Structural Biology
    • Biophysics
    • Biochemistry
    • Genetics

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