Complete thrombospondin mRNA sequence includes potential regulatory sites in the 3' untranslated region

S. W. Hennessy, B. A. Frazier, D. D. Kim, T. L. Deckwerth, D. M. Baumgartel, P. Rotwein, W. A. Frazier

Research output: Contribution to journalArticle

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Abstract

The nucleotide sequence of human thrombospondin (TS) mRNA has been determined from human fibroblast and endothelial cDNAs. The sequence of 5802 bp begins 110 bp upstream from the initiator codon and includes the entire 3' untranslated region (UTR) of the mRNA. The coding region (3510 bp) specifies a protein of 1170 amino acids with all of the known features of the TS subunit (Frazier, W.A. 1987. J. Cell Biol. 105:625-632). The long 3' UTR of 2166 nucleotides is extremely A/T-rich, particularly in the latter half. It contains 37 TATT or ATTT(A) sequences that have been suggested as mediators of the stability of mRNAs for cytokines, lymphokines, and oncogenes (Shaw, G., and R. Kamen. 1986. Cell. 46:659-667). Another unusual feature of the 3' UTR of TS mRNA is a stretch of 42 nucleotides of which 40 are thymidines (uridine in the mRNA) including an uninterrupted sequence of 26 thymidines. This region is flanked by two sets of direct repeats suggesting that it may be an insertion element of retrotranscriptional origin. Comparison of the 3' untranslated region of TS mRNA with the GenBank data base indicates the greatest degree of similarity with an α-interferon gene which contains a number of the TATT/ATTT consensus sites. The degree of similarity between the TS and interferon sequences is the same in regions of the interferon gene corresponding to its coding and non-coding regions suggesting that most of the TS 3' UTR may be derived from an interferon gene of pseudogene. The features of the TS mRNA 3' UTR provide a potential explanation for the rapid regulation of TS message observed in cultured cells in response to PDGF and sugest that TS is a member of a group of proteins which are intimately involved in the control of cell growth and differentiation.

Original languageEnglish (US)
Pages (from-to)729-736
Number of pages8
JournalJournal of Cell Biology
Volume108
Issue number2
StatePublished - 1989
Externally publishedYes

Fingerprint

Thrombospondins
3' Untranslated Regions
Messenger RNA
Interferons
Thymidine
Nucleotides
Genes
Pseudogenes
Initiator Codon
Lymphokines
Nucleic Acid Repetitive Sequences
Uridine
Nucleic Acid Databases
RNA Stability
Oncogenes
Cell Differentiation
Cultured Cells
Proteins
Complementary DNA
Fibroblasts

ASJC Scopus subject areas

  • Cell Biology

Cite this

Hennessy, S. W., Frazier, B. A., Kim, D. D., Deckwerth, T. L., Baumgartel, D. M., Rotwein, P., & Frazier, W. A. (1989). Complete thrombospondin mRNA sequence includes potential regulatory sites in the 3' untranslated region. Journal of Cell Biology, 108(2), 729-736.

Complete thrombospondin mRNA sequence includes potential regulatory sites in the 3' untranslated region. / Hennessy, S. W.; Frazier, B. A.; Kim, D. D.; Deckwerth, T. L.; Baumgartel, D. M.; Rotwein, P.; Frazier, W. A.

In: Journal of Cell Biology, Vol. 108, No. 2, 1989, p. 729-736.

Research output: Contribution to journalArticle

Hennessy, SW, Frazier, BA, Kim, DD, Deckwerth, TL, Baumgartel, DM, Rotwein, P & Frazier, WA 1989, 'Complete thrombospondin mRNA sequence includes potential regulatory sites in the 3' untranslated region', Journal of Cell Biology, vol. 108, no. 2, pp. 729-736.
Hennessy SW, Frazier BA, Kim DD, Deckwerth TL, Baumgartel DM, Rotwein P et al. Complete thrombospondin mRNA sequence includes potential regulatory sites in the 3' untranslated region. Journal of Cell Biology. 1989;108(2):729-736.
Hennessy, S. W. ; Frazier, B. A. ; Kim, D. D. ; Deckwerth, T. L. ; Baumgartel, D. M. ; Rotwein, P. ; Frazier, W. A. / Complete thrombospondin mRNA sequence includes potential regulatory sites in the 3' untranslated region. In: Journal of Cell Biology. 1989 ; Vol. 108, No. 2. pp. 729-736.
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abstract = "The nucleotide sequence of human thrombospondin (TS) mRNA has been determined from human fibroblast and endothelial cDNAs. The sequence of 5802 bp begins 110 bp upstream from the initiator codon and includes the entire 3' untranslated region (UTR) of the mRNA. The coding region (3510 bp) specifies a protein of 1170 amino acids with all of the known features of the TS subunit (Frazier, W.A. 1987. J. Cell Biol. 105:625-632). The long 3' UTR of 2166 nucleotides is extremely A/T-rich, particularly in the latter half. It contains 37 TATT or ATTT(A) sequences that have been suggested as mediators of the stability of mRNAs for cytokines, lymphokines, and oncogenes (Shaw, G., and R. Kamen. 1986. Cell. 46:659-667). Another unusual feature of the 3' UTR of TS mRNA is a stretch of 42 nucleotides of which 40 are thymidines (uridine in the mRNA) including an uninterrupted sequence of 26 thymidines. This region is flanked by two sets of direct repeats suggesting that it may be an insertion element of retrotranscriptional origin. Comparison of the 3' untranslated region of TS mRNA with the GenBank data base indicates the greatest degree of similarity with an α-interferon gene which contains a number of the TATT/ATTT consensus sites. The degree of similarity between the TS and interferon sequences is the same in regions of the interferon gene corresponding to its coding and non-coding regions suggesting that most of the TS 3' UTR may be derived from an interferon gene of pseudogene. The features of the TS mRNA 3' UTR provide a potential explanation for the rapid regulation of TS message observed in cultured cells in response to PDGF and sugest that TS is a member of a group of proteins which are intimately involved in the control of cell growth and differentiation.",
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