Complete sequence and genomic analysis of rhesus cytomegalovirus

Scott Hansen, Lisa I. Strelow, David C. Franchi, David G. Anders, Scott Wong

Research output: Contribution to journalArticle

130 Citations (Scopus)

Abstract

The complete DNA sequence of rhesus cytomegalovirus (RhCMV) strain 68-1 was determined with the whole-genome shotgun approach on virion DNA. The RhCMV genome is 221,459 bp in length and possesses a 49% G+C base composition. The genome contains 230 potential open reading frames (ORFs) of 100 or more codons that are arranged colinearly with counterparts of previously sequenced betaherpesviruses such as human cytomegalovirus (HCMV). Of the 230 RhCMV ORFs, 138 (60%) are homologous to known HCMV proteins. The conserved ORFs include the structural, replicative, and transcriptional regulatory proteins, immune evasion elements, G protein-coupled receptors, and immunoglobulin homologues. Interestingly, the RhCMV genome also contains sequences with homology to cyclooxygenase-2, an enzyme associated with inflammatory processes. Closer examination identified a series of candidate exons with the capacity to encode a full-length cyclooxygenase-2 protein. Counterparts of cyclooxygenase-2 have not been found in other sequenced herpesviruses. The availability of the complete RhCMV sequence along with the ability to grow RhCMV in vitro will facilitate the construction of recombinant viral strains for identifying viral determinants of CMV pathogenicity in the experimentally infected rhesus macaque and to the development of CMV as a vaccine vector.

Original languageEnglish (US)
Pages (from-to)6620-6636
Number of pages17
JournalJournal of Virology
Volume77
Issue number12
DOIs
StatePublished - Jun 2003

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Cytomegalovirus
Sequence Analysis
sequence analysis
genomics
prostaglandin synthase
Human herpesvirus 5
open reading frames
Cyclooxygenase 2
genome
Genome
Open Reading Frames
Base Composition
immune evasion
vector vaccines
regulatory proteins
Macaca mulatta
Immune Evasion
virion
codons
immunoglobulins

ASJC Scopus subject areas

  • Immunology

Cite this

Complete sequence and genomic analysis of rhesus cytomegalovirus. / Hansen, Scott; Strelow, Lisa I.; Franchi, David C.; Anders, David G.; Wong, Scott.

In: Journal of Virology, Vol. 77, No. 12, 06.2003, p. 6620-6636.

Research output: Contribution to journalArticle

Hansen, Scott ; Strelow, Lisa I. ; Franchi, David C. ; Anders, David G. ; Wong, Scott. / Complete sequence and genomic analysis of rhesus cytomegalovirus. In: Journal of Virology. 2003 ; Vol. 77, No. 12. pp. 6620-6636.
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