Complete Amino Acid Sequence of the Precursor Region of Rat Prolactin

David J. McKean; Richard A. Maurer

doi:10.1021/bi00617a022

Complete Amino Acid Sequence of the Precursor Region of Rat Prolactin

David J. McKean, Richard A. Maurer

Cell, Developmental, & Cancer Biology

Research output: Contribution to journal › Article › peer-review

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Abstract

Rat preprolactin was translated in a wheat germ in vitro synthetic system in the presence of 3H-labeled amino acids. The amino-terminal sequence of the radiolabeled precursor was quantitatively identified by high pressure liquid chromatography. The 29 residue precursor sequence is: Met-Asn-Ser-Gln-Val-Ser-Ala-Arg-Lys-Ala-Gly-Thr-Leu-Leu-Leu-Leu-Met-Met-Ser-Asn-Leu-Leu-Phe-Cys-Gln-Asn-Val-Gln-Thr-. A discussion of the structure-function relationships of the precursor region and the microsequencing methods used to determine the sequence is presented. The amino-terminal 30 residue sequence of the secreted form of rat prolactin was determined and found to differ at positions 7 and 8 (-Gly-Asp-) from a previously reported rat prolactin sequence (Parlow, A. F., & Shome, B. (1976) Fed. Proc., Fed. Am. Soc. Exp. Biol. 35, 219).

Original language	English (US)
Pages (from-to)	5215-5219
Number of pages	5
Journal	Biochemistry
Volume	17
Issue number	24
DOIs	https://doi.org/10.1021/bi00617a022
State	Published - Jan 1 1978

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Biochemistry

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title = "Complete Amino Acid Sequence of the Precursor Region of Rat Prolactin",

abstract = "Rat preprolactin was translated in a wheat germ in vitro synthetic system in the presence of 3H-labeled amino acids. The amino-terminal sequence of the radiolabeled precursor was quantitatively identified by high pressure liquid chromatography. The 29 residue precursor sequence is: Met-Asn-Ser-Gln-Val-Ser-Ala-Arg-Lys-Ala-Gly-Thr-Leu-Leu-Leu-Leu-Met-Met-Ser-Asn-Leu-Leu-Phe-Cys-Gln-Asn-Val-Gln-Thr-. A discussion of the structure-function relationships of the precursor region and the microsequencing methods used to determine the sequence is presented. The amino-terminal 30 residue sequence of the secreted form of rat prolactin was determined and found to differ at positions 7 and 8 (-Gly-Asp-) from a previously reported rat prolactin sequence (Parlow, A. F., & Shome, B. (1976) Fed. Proc., Fed. Am. Soc. Exp. Biol. 35, 219).",

author = "McKean, {David J.} and Maurer, {Richard A.}",

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T1 - Complete Amino Acid Sequence of the Precursor Region of Rat Prolactin

AU - McKean, David J.

AU - Maurer, Richard A.

PY - 1978/1/1

Y1 - 1978/1/1

N2 - Rat preprolactin was translated in a wheat germ in vitro synthetic system in the presence of 3H-labeled amino acids. The amino-terminal sequence of the radiolabeled precursor was quantitatively identified by high pressure liquid chromatography. The 29 residue precursor sequence is: Met-Asn-Ser-Gln-Val-Ser-Ala-Arg-Lys-Ala-Gly-Thr-Leu-Leu-Leu-Leu-Met-Met-Ser-Asn-Leu-Leu-Phe-Cys-Gln-Asn-Val-Gln-Thr-. A discussion of the structure-function relationships of the precursor region and the microsequencing methods used to determine the sequence is presented. The amino-terminal 30 residue sequence of the secreted form of rat prolactin was determined and found to differ at positions 7 and 8 (-Gly-Asp-) from a previously reported rat prolactin sequence (Parlow, A. F., & Shome, B. (1976) Fed. Proc., Fed. Am. Soc. Exp. Biol. 35, 219).

AB - Rat preprolactin was translated in a wheat germ in vitro synthetic system in the presence of 3H-labeled amino acids. The amino-terminal sequence of the radiolabeled precursor was quantitatively identified by high pressure liquid chromatography. The 29 residue precursor sequence is: Met-Asn-Ser-Gln-Val-Ser-Ala-Arg-Lys-Ala-Gly-Thr-Leu-Leu-Leu-Leu-Met-Met-Ser-Asn-Leu-Leu-Phe-Cys-Gln-Asn-Val-Gln-Thr-. A discussion of the structure-function relationships of the precursor region and the microsequencing methods used to determine the sequence is presented. The amino-terminal 30 residue sequence of the secreted form of rat prolactin was determined and found to differ at positions 7 and 8 (-Gly-Asp-) from a previously reported rat prolactin sequence (Parlow, A. F., & Shome, B. (1976) Fed. Proc., Fed. Am. Soc. Exp. Biol. 35, 219).

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JO - Biochemistry

JF - Biochemistry

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ER -

Complete Amino Acid Sequence of the Precursor Region of Rat Prolactin

Abstract

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