Competition and Caries on Enamel of a Dual-Species Biofilm Model with Streptococcus mutans and Streptococcus sanguinis

Natalia Díaz-Garrido, Carla P. Lozano, Jens Kreth, Rodrigo A. Giacaman

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Imbalances within the dental biofilm trigger dental caries, currently consid- ered a dysbiosis and the most prevalent noncommunicable disease. There is still a gap in knowledge about the dynamics of enamel colonization by bacteria from the dental biofilm in caries. The aim, therefore, was to test whether the sequence of enamel coloni-zation by a typically commensal and a cariogenic species modifies biofilm's cariogenicity. Dual-species biofilms of Streptococcus mutans and Streptococcus sanguinis on saliva-coated enamel slabs were inoculated in different sequences: S. mutans followed by S. sanguinis (Sm-Ss), S. sanguinis followed by S. mutans (Ss-Sm), S. mutans and S. sanguinis inoculated at the same time (Sm=Ss), and the single-species controls S. mutans followed by S. mutans (Sm-Sm) and S. sanguinis followed by S. sanguinis (Ss-Ss). Biofilms were ex-posed to 10% sucrose 3 times per day for 5 days, and the slabs/biofilms were retrieved to assess demineralization, viable cells, biomass, proteins, polysaccharides, and H202 pro-duction. Compared with Sm-Sm, primary inoculation with S. sanguinis reduced deminer-alization (P < 0.05). Both Ss-Sm and Sm=Ss sequences showed reduction in biomass, protein, and polysaccharide content (P < 0.05). The highest S. sanguinis viable count and H202 production level and the lowest acidogenicity were observed when S. sanguinis colonized enamel before S. mutans (P < 0.05). Initial enamel adherence with commensal biofilms seems to induce more intense competition against more typically cariogenic species, reducing cariogenicity.

Original languageEnglish (US)
Article numbere01262-20
JournalApplied and Environmental Microbiology
Volume86
Issue number21
DOIs
StatePublished - Nov 2020

Keywords

  • Streptococcus mutans
  • Streptococcus sanguinis
  • cariogenicity
  • colonization
  • competition
  • dental caries
  • oral biofilm
  • primary

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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