Comparison of the UV resonance Raman spectra of bacteria, bacterial cell walls, and ribosomes excited in the deep UV

S. Chadha, R. Manoharan, Pierre Moenne-Loccoz, W. H. Nelson, W. L. Peticolas, J. F. Sperry

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Resonance Raman spectra have been obtained with 218-nm excitation for Escherichia coli and Staphylococcus epidermidis. Large intensity differences seen in the tryptophan-associated 1556-cm-1 peak appear to be strongly related to Gram type. Unlike E. coli, S. epidermidis possess a very intense peak at 1658 cm-1 which varies in intensity with cultural conditions. Spectra excited from E. coli and Enterobacter aerogenes with 200-nm light show peaks which strongly reflect nucleic acid composition, unlike spectra excited at 218 nm. Purified, separated ribosomes of E. coli produce spectra which are dominated by nucleic acid vibrations when excited at 242 nm, but have peaks belonging nearly exclusively to protein aromatic amino acids when excited at 222 nm. The relative weakness of bacterial RNA modes excited at 222 nm from whole cells and ribosomes is attributed to nucleic acid hypochromism.

Original languageEnglish (US)
Pages (from-to)38-43
Number of pages6
JournalApplied Spectroscopy
Volume47
Issue number1
DOIs
StatePublished - 1993
Externally publishedYes

Fingerprint

ribosomes
Escherichia coli
bacteria
Raman scattering
Nucleic acids
Bacteria
nucleic acids
Cells
Raman spectra
Nucleic Acids
Bacterial RNA
Aromatic Amino Acids
staphylococcus
tryptophan
Escherichia
RNA
Carboxylic acids
Tryptophan
amino acids
Amino acids

Keywords

  • Analytical methods
  • Raman spectroscopy
  • Spectroscopic techniques

ASJC Scopus subject areas

  • Spectroscopy
  • Instrumentation

Cite this

Comparison of the UV resonance Raman spectra of bacteria, bacterial cell walls, and ribosomes excited in the deep UV. / Chadha, S.; Manoharan, R.; Moenne-Loccoz, Pierre; Nelson, W. H.; Peticolas, W. L.; Sperry, J. F.

In: Applied Spectroscopy, Vol. 47, No. 1, 1993, p. 38-43.

Research output: Contribution to journalArticle

Chadha, S. ; Manoharan, R. ; Moenne-Loccoz, Pierre ; Nelson, W. H. ; Peticolas, W. L. ; Sperry, J. F. / Comparison of the UV resonance Raman spectra of bacteria, bacterial cell walls, and ribosomes excited in the deep UV. In: Applied Spectroscopy. 1993 ; Vol. 47, No. 1. pp. 38-43.
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AU - Sperry, J. F.

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