Comparison of PDT efficiencies for photooxidation of substrate (NADPH) using a photosensitizer (Photofrin II)

Paulo R. Bargo, Parmeswaran Diagaradjane, Steven Jacques

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Photooxidation was studied during PDT (Photodynamic therapy) in a model system using NADPH as the target substrate and Photofrin II as the photosensitizer. The efficiency of NADPH oxidation per photon absorbed by photosensitizer was determined as a function of substrate concentration. The kinetics of changes in absorbance (340 nm) was measured to assay oxidation of NADPH. The efficiency of oxidation increased (.002; .004: .0049; .005) with increasing NADPH concentration (0.4; 1; 3.5; 10 mM) and approached a stable value of 0.0048. Using typical values for the quantum efficiency of the Photofrin triplet state generation and the efficiency of singlet oxygen production we obtained a value for the efficiency of interaction between singlet oxygen and NADPH (0.024). Efficiency of diffusion of singlet oxygen was modeled with molecular diffusion theory using typical values for the singlet oxygen lifetime and the diffusion coefficient in aqueous solutions.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherSociety of Photo-Optical Instrumentation Engineers
Pages97-103
Number of pages7
Volume3909
StatePublished - 2000
EventOptical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy IX - San Jose, CA, USA
Duration: Jan 22 2000Jan 23 2000

Other

OtherOptical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy IX
CitySan Jose, CA, USA
Period1/22/001/23/00

Fingerprint

Photodynamic therapy
Photosensitizers
Photooxidation
photooxidation
therapy
Oxygen
Substrates
Oxidation
oxidation
oxygen
oxygen production
Quantum efficiency
molecular diffusion
diffusion theory
Assays
Photons
atomic energy levels
quantum efficiency
diffusion coefficient
Kinetics

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Bargo, P. R., Diagaradjane, P., & Jacques, S. (2000). Comparison of PDT efficiencies for photooxidation of substrate (NADPH) using a photosensitizer (Photofrin II). In Proceedings of SPIE - The International Society for Optical Engineering (Vol. 3909, pp. 97-103). Society of Photo-Optical Instrumentation Engineers.

Comparison of PDT efficiencies for photooxidation of substrate (NADPH) using a photosensitizer (Photofrin II). / Bargo, Paulo R.; Diagaradjane, Parmeswaran; Jacques, Steven.

Proceedings of SPIE - The International Society for Optical Engineering. Vol. 3909 Society of Photo-Optical Instrumentation Engineers, 2000. p. 97-103.

Research output: Chapter in Book/Report/Conference proceedingChapter

Bargo, PR, Diagaradjane, P & Jacques, S 2000, Comparison of PDT efficiencies for photooxidation of substrate (NADPH) using a photosensitizer (Photofrin II). in Proceedings of SPIE - The International Society for Optical Engineering. vol. 3909, Society of Photo-Optical Instrumentation Engineers, pp. 97-103, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy IX, San Jose, CA, USA, 1/22/00.
Bargo PR, Diagaradjane P, Jacques S. Comparison of PDT efficiencies for photooxidation of substrate (NADPH) using a photosensitizer (Photofrin II). In Proceedings of SPIE - The International Society for Optical Engineering. Vol. 3909. Society of Photo-Optical Instrumentation Engineers. 2000. p. 97-103
Bargo, Paulo R. ; Diagaradjane, Parmeswaran ; Jacques, Steven. / Comparison of PDT efficiencies for photooxidation of substrate (NADPH) using a photosensitizer (Photofrin II). Proceedings of SPIE - The International Society for Optical Engineering. Vol. 3909 Society of Photo-Optical Instrumentation Engineers, 2000. pp. 97-103
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