Comparison of metabolic profiles and bioactivities of the leaves of three edible Congolese Hibiscus species

Paulin Mutwale Kapepula, Nadege Kabamba Ngombe, Pascal Tshisekedi Tshibangu, César Tsumbu, Thierry Franck, Ange Mouithys-Mickalad, Dieudonné Mumba, Daniel Tshala-Katumbay, Didier Serteyn, Monique Tits, Luc Angenot, Pascal Dibungi T Kalenda, Michel Frédérich

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Methanolic and dichloromethane extracts from the leaves of Congolese Hibiscus species were characterised by chromatographic and spectroscopic techniques and their in vitro biochemical activities against ROS production were evaluated in cellular models and on an enzyme, myeloperoxidase (MPO), involved in inflammation. Hibiscus acetosella has a chemical fingerprint different from Hibiscus cannabinus and Hibiscus sabdariffa both having similar fingerprints. Major compounds were polyphenols, represented mainly by caffeoyl-hydroxycitric acid for H. acetosella and neochlorogenic acid for the two other species. All extracts displayed high cellular antioxidant activity with IC50 values ranging from 0.5 to 3 μg mL−1 using lucigenin on neutrophils. Dichloromethane extracts showed more efficient effects on extracellular ROS production and MPO activity. Antioxidant and anti-inflammatory activities of caffeoyl-hydroxycitric acid were significantly higher than those of neochlorogenic acid. The bioactivities of Hibiscus species were positively correlated with their phytochemical content and could justify their use as local nutraceutical resources and medicines.

Original languageEnglish (US)
Pages (from-to)1-8
Number of pages8
JournalNatural Product Research
DOIs
StateAccepted/In press - Mar 19 2017

Fingerprint

Hibiscus
Metabolome
Methylene Chloride
Bioactivity
Peroxidase
Hibiscus acetosella
Antioxidants
myeloperoxidase
acids
Phytochemicals
Polyphenols
methylene chloride
Medicine
Dermatoglyphics
leaves
extracts
Anti-Inflammatory Agents
Hibiscus cannabinus
Hibiscus sabdariffa
Enzymes

Keywords

  • Caffeoyl-hydroxycitric acid
  • Hibiscus acetosella
  • Hibiscus cannabinus
  • Hibiscus sabdariffa
  • myeloperoxidase
  • neochlorogenic acid

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Plant Science
  • Organic Chemistry

Cite this

Kapepula, P. M., Kabamba Ngombe, N., Tshisekedi Tshibangu, P., Tsumbu, C., Franck, T., Mouithys-Mickalad, A., ... Frédérich, M. (Accepted/In press). Comparison of metabolic profiles and bioactivities of the leaves of three edible Congolese Hibiscus species. Natural Product Research, 1-8. https://doi.org/10.1080/14786419.2017.1305382

Comparison of metabolic profiles and bioactivities of the leaves of three edible Congolese Hibiscus species. / Kapepula, Paulin Mutwale; Kabamba Ngombe, Nadege; Tshisekedi Tshibangu, Pascal; Tsumbu, César; Franck, Thierry; Mouithys-Mickalad, Ange; Mumba, Dieudonné; Tshala-Katumbay, Daniel; Serteyn, Didier; Tits, Monique; Angenot, Luc; Kalenda, Pascal Dibungi T; Frédérich, Michel.

In: Natural Product Research, 19.03.2017, p. 1-8.

Research output: Contribution to journalArticle

Kapepula, PM, Kabamba Ngombe, N, Tshisekedi Tshibangu, P, Tsumbu, C, Franck, T, Mouithys-Mickalad, A, Mumba, D, Tshala-Katumbay, D, Serteyn, D, Tits, M, Angenot, L, Kalenda, PDT & Frédérich, M 2017, 'Comparison of metabolic profiles and bioactivities of the leaves of three edible Congolese Hibiscus species', Natural Product Research, pp. 1-8. https://doi.org/10.1080/14786419.2017.1305382
Kapepula PM, Kabamba Ngombe N, Tshisekedi Tshibangu P, Tsumbu C, Franck T, Mouithys-Mickalad A et al. Comparison of metabolic profiles and bioactivities of the leaves of three edible Congolese Hibiscus species. Natural Product Research. 2017 Mar 19;1-8. https://doi.org/10.1080/14786419.2017.1305382
Kapepula, Paulin Mutwale ; Kabamba Ngombe, Nadege ; Tshisekedi Tshibangu, Pascal ; Tsumbu, César ; Franck, Thierry ; Mouithys-Mickalad, Ange ; Mumba, Dieudonné ; Tshala-Katumbay, Daniel ; Serteyn, Didier ; Tits, Monique ; Angenot, Luc ; Kalenda, Pascal Dibungi T ; Frédérich, Michel. / Comparison of metabolic profiles and bioactivities of the leaves of three edible Congolese Hibiscus species. In: Natural Product Research. 2017 ; pp. 1-8.
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