Comparison of lysis-centrifugation with lysis-filtration and a conventional unvented bottle for blood cultures

V. J. Gill, C. H. Zierdt, T. C. Wu, F. Stock, P. A. Pizzo, James Maclowry

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Evaluation of a commercially available lysis-centrifugation blood culture system (Isolator, DuPont Co., Wilmington, Del.) and a lysis-filtration blood culture system for 3,111 cultures showed that both methods had comparable recoveries (73 and 68%, respectively) of significant aerobic and facultatively anaerobic isolates. The unvented conventional blood culture bottle had a recovery rate of 59%. Although the lysis-centrifugation and lysis-filtration systems had comparable recoveries of pathogens, the lysis-centrifugation system had the advantage of having colonies immediately available for further testing. The contamination rate with the lysis-centrifugation system was 3%, compared with 6% with the lysis-filtration system and 0.4% with brain heart infusion.

Original languageEnglish (US)
Pages (from-to)927-932
Number of pages6
JournalJournal of Clinical Microbiology
Volume20
Issue number5
StatePublished - 1984
Externally publishedYes

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Centrifugation
Blood Culture
Brain

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Comparison of lysis-centrifugation with lysis-filtration and a conventional unvented bottle for blood cultures. / Gill, V. J.; Zierdt, C. H.; Wu, T. C.; Stock, F.; Pizzo, P. A.; Maclowry, James.

In: Journal of Clinical Microbiology, Vol. 20, No. 5, 1984, p. 927-932.

Research output: Contribution to journalArticle

Gill, V. J. ; Zierdt, C. H. ; Wu, T. C. ; Stock, F. ; Pizzo, P. A. ; Maclowry, James. / Comparison of lysis-centrifugation with lysis-filtration and a conventional unvented bottle for blood cultures. In: Journal of Clinical Microbiology. 1984 ; Vol. 20, No. 5. pp. 927-932.
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