Comparison of intracanal endosequence root repair material and proroot MTA to induce pH changes in simulated root resorption defects over 4 weeks in matched pairs of human teeth

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Introduction: Intracanal mineral trioxide aggregate (MTA) may provide an alternative to calcium hydroxide in the treatment of external inflammatory root resorption. This in vitro study using human matched pairs of teeth compared white ProRoot MTA (WMTA; (Dentsply Tulsa Dental Specialties, Tulsa, OK) and an alternative material with purportedly improved handling properties, EndoSequence Root Repair Material (ES; Brasseler USA, Savannah, GA), by measuring pH in simulated root surface resorptive defects after intracanal placement. The null hypothesis tested was that there is no difference between WMTA and ES. Methods: Bilaterally matched pairs (n = 24) of extracted, human, single-rooted teeth were instrumented to apical size 50/.06, and root surface cavities were prepared at 5 mm and 2 mm from the apex. Root canals of experimental matched pairs (n = 20) were filled with WMTA or ES; control pairs (n = 4) were filled with calcium hydroxide (positive control [POS]) or saline (negative control [NEG]). Teeth were sealed coronally and apically and immersed in saline. The pH in root surface cavities was measured at 20 minutes, 3 hours, 24 hours, 1 week, 2 weeks, 3 weeks, and 4 weeks. Results: The pH at 5 mm when compared with the 2-mm level was significantly higher for the WMTA, ES, and POS groups (P <.05, paired t tests); therefore, each level was analyzed separately. At both the 2-mm and 5-mm levels, significant pH changes occurred over time in the WMTA, ES (both P <.0001, repeated-measures analysis of variance), and POS (P <.0001, Friedman test) groups and not in the NEG group (mean pH = 7.32 ± 0.04, P > .05). There were no differences between WMTA and ES at 20 minutes and 3 hours at both levels or at 24 hours at 5mm. The pH of WMTA was higher than ES by 24 hours at the 2-mm level (8.79 vs 8.56, P <.05, paired t test) and after 1 week at the 5-mm level (8.91 vs 8.05, P <.0001) and was thereafter always significantly higher in WMTA compared with ES (P <.0001). The null hypothesis was rejected. Conclusions: In matched pairs of teeth, intracanal placement of WMTA compared with ES resulted in a higher pH in simulated root resorption defects that was time and root level dependent.

Original languageEnglish (US)
Pages (from-to)502-506
Number of pages5
JournalJournal of Endodontics
Volume37
Issue number4
DOIs
StatePublished - Apr 2011

Fingerprint

Root Resorption
Tooth
Calcium Hydroxide
Dental Specialties
Dental Pulp Cavity
mineral trioxide aggregate
EndoSequence root repair material
Control Groups

Keywords

  • 4 weeks
  • calcium hydroxide
  • dentin
  • diffusion
  • EndoSequence Root Repair Material
  • human
  • hydroxyl ion
  • in vitro
  • matched pairs
  • mineral trioxide aggregate
  • pH
  • pH changes
  • ProRoot 14 MTA
  • root canal
  • root resorption
  • root surface cavities
  • saline
  • simulated root resorption defects
  • teeth

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

@article{c1b1af607a1f4feeb521d093830ff5be,
title = "Comparison of intracanal endosequence root repair material and proroot MTA to induce pH changes in simulated root resorption defects over 4 weeks in matched pairs of human teeth",
abstract = "Introduction: Intracanal mineral trioxide aggregate (MTA) may provide an alternative to calcium hydroxide in the treatment of external inflammatory root resorption. This in vitro study using human matched pairs of teeth compared white ProRoot MTA (WMTA; (Dentsply Tulsa Dental Specialties, Tulsa, OK) and an alternative material with purportedly improved handling properties, EndoSequence Root Repair Material (ES; Brasseler USA, Savannah, GA), by measuring pH in simulated root surface resorptive defects after intracanal placement. The null hypothesis tested was that there is no difference between WMTA and ES. Methods: Bilaterally matched pairs (n = 24) of extracted, human, single-rooted teeth were instrumented to apical size 50/.06, and root surface cavities were prepared at 5 mm and 2 mm from the apex. Root canals of experimental matched pairs (n = 20) were filled with WMTA or ES; control pairs (n = 4) were filled with calcium hydroxide (positive control [POS]) or saline (negative control [NEG]). Teeth were sealed coronally and apically and immersed in saline. The pH in root surface cavities was measured at 20 minutes, 3 hours, 24 hours, 1 week, 2 weeks, 3 weeks, and 4 weeks. Results: The pH at 5 mm when compared with the 2-mm level was significantly higher for the WMTA, ES, and POS groups (P <.05, paired t tests); therefore, each level was analyzed separately. At both the 2-mm and 5-mm levels, significant pH changes occurred over time in the WMTA, ES (both P <.0001, repeated-measures analysis of variance), and POS (P <.0001, Friedman test) groups and not in the NEG group (mean pH = 7.32 ± 0.04, P > .05). There were no differences between WMTA and ES at 20 minutes and 3 hours at both levels or at 24 hours at 5mm. The pH of WMTA was higher than ES by 24 hours at the 2-mm level (8.79 vs 8.56, P <.05, paired t test) and after 1 week at the 5-mm level (8.91 vs 8.05, P <.0001) and was thereafter always significantly higher in WMTA compared with ES (P <.0001). The null hypothesis was rejected. Conclusions: In matched pairs of teeth, intracanal placement of WMTA compared with ES resulted in a higher pH in simulated root resorption defects that was time and root level dependent.",
keywords = "4 weeks, calcium hydroxide, dentin, diffusion, EndoSequence Root Repair Material, human, hydroxyl ion, in vitro, matched pairs, mineral trioxide aggregate, pH, pH changes, ProRoot 14 MTA, root canal, root resorption, root surface cavities, saline, simulated root resorption defects, teeth",
author = "Hansen, {Stephen W.} and Marshall, {J (Gordon)} and Christine Sedgley",
year = "2011",
month = "4",
doi = "10.1016/j.joen.2011.01.010",
language = "English (US)",
volume = "37",
pages = "502--506",
journal = "Journal of Endodontics",
issn = "0099-2399",
publisher = "Elsevier Inc.",
number = "4",

}

TY - JOUR

T1 - Comparison of intracanal endosequence root repair material and proroot MTA to induce pH changes in simulated root resorption defects over 4 weeks in matched pairs of human teeth

AU - Hansen, Stephen W.

AU - Marshall, J (Gordon)

AU - Sedgley, Christine

PY - 2011/4

Y1 - 2011/4

N2 - Introduction: Intracanal mineral trioxide aggregate (MTA) may provide an alternative to calcium hydroxide in the treatment of external inflammatory root resorption. This in vitro study using human matched pairs of teeth compared white ProRoot MTA (WMTA; (Dentsply Tulsa Dental Specialties, Tulsa, OK) and an alternative material with purportedly improved handling properties, EndoSequence Root Repair Material (ES; Brasseler USA, Savannah, GA), by measuring pH in simulated root surface resorptive defects after intracanal placement. The null hypothesis tested was that there is no difference between WMTA and ES. Methods: Bilaterally matched pairs (n = 24) of extracted, human, single-rooted teeth were instrumented to apical size 50/.06, and root surface cavities were prepared at 5 mm and 2 mm from the apex. Root canals of experimental matched pairs (n = 20) were filled with WMTA or ES; control pairs (n = 4) were filled with calcium hydroxide (positive control [POS]) or saline (negative control [NEG]). Teeth were sealed coronally and apically and immersed in saline. The pH in root surface cavities was measured at 20 minutes, 3 hours, 24 hours, 1 week, 2 weeks, 3 weeks, and 4 weeks. Results: The pH at 5 mm when compared with the 2-mm level was significantly higher for the WMTA, ES, and POS groups (P <.05, paired t tests); therefore, each level was analyzed separately. At both the 2-mm and 5-mm levels, significant pH changes occurred over time in the WMTA, ES (both P <.0001, repeated-measures analysis of variance), and POS (P <.0001, Friedman test) groups and not in the NEG group (mean pH = 7.32 ± 0.04, P > .05). There were no differences between WMTA and ES at 20 minutes and 3 hours at both levels or at 24 hours at 5mm. The pH of WMTA was higher than ES by 24 hours at the 2-mm level (8.79 vs 8.56, P <.05, paired t test) and after 1 week at the 5-mm level (8.91 vs 8.05, P <.0001) and was thereafter always significantly higher in WMTA compared with ES (P <.0001). The null hypothesis was rejected. Conclusions: In matched pairs of teeth, intracanal placement of WMTA compared with ES resulted in a higher pH in simulated root resorption defects that was time and root level dependent.

AB - Introduction: Intracanal mineral trioxide aggregate (MTA) may provide an alternative to calcium hydroxide in the treatment of external inflammatory root resorption. This in vitro study using human matched pairs of teeth compared white ProRoot MTA (WMTA; (Dentsply Tulsa Dental Specialties, Tulsa, OK) and an alternative material with purportedly improved handling properties, EndoSequence Root Repair Material (ES; Brasseler USA, Savannah, GA), by measuring pH in simulated root surface resorptive defects after intracanal placement. The null hypothesis tested was that there is no difference between WMTA and ES. Methods: Bilaterally matched pairs (n = 24) of extracted, human, single-rooted teeth were instrumented to apical size 50/.06, and root surface cavities were prepared at 5 mm and 2 mm from the apex. Root canals of experimental matched pairs (n = 20) were filled with WMTA or ES; control pairs (n = 4) were filled with calcium hydroxide (positive control [POS]) or saline (negative control [NEG]). Teeth were sealed coronally and apically and immersed in saline. The pH in root surface cavities was measured at 20 minutes, 3 hours, 24 hours, 1 week, 2 weeks, 3 weeks, and 4 weeks. Results: The pH at 5 mm when compared with the 2-mm level was significantly higher for the WMTA, ES, and POS groups (P <.05, paired t tests); therefore, each level was analyzed separately. At both the 2-mm and 5-mm levels, significant pH changes occurred over time in the WMTA, ES (both P <.0001, repeated-measures analysis of variance), and POS (P <.0001, Friedman test) groups and not in the NEG group (mean pH = 7.32 ± 0.04, P > .05). There were no differences between WMTA and ES at 20 minutes and 3 hours at both levels or at 24 hours at 5mm. The pH of WMTA was higher than ES by 24 hours at the 2-mm level (8.79 vs 8.56, P <.05, paired t test) and after 1 week at the 5-mm level (8.91 vs 8.05, P <.0001) and was thereafter always significantly higher in WMTA compared with ES (P <.0001). The null hypothesis was rejected. Conclusions: In matched pairs of teeth, intracanal placement of WMTA compared with ES resulted in a higher pH in simulated root resorption defects that was time and root level dependent.

KW - 4 weeks

KW - calcium hydroxide

KW - dentin

KW - diffusion

KW - EndoSequence Root Repair Material

KW - human

KW - hydroxyl ion

KW - in vitro

KW - matched pairs

KW - mineral trioxide aggregate

KW - pH

KW - pH changes

KW - ProRoot 14 MTA

KW - root canal

KW - root resorption

KW - root surface cavities

KW - saline

KW - simulated root resorption defects

KW - teeth

UR - http://www.scopus.com/inward/record.url?scp=79952785697&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79952785697&partnerID=8YFLogxK

U2 - 10.1016/j.joen.2011.01.010

DO - 10.1016/j.joen.2011.01.010

M3 - Article

C2 - 21419298

AN - SCOPUS:79952785697

VL - 37

SP - 502

EP - 506

JO - Journal of Endodontics

JF - Journal of Endodontics

SN - 0099-2399

IS - 4

ER -