Comparison of epigenetic mediator expression and function in mouse and human embryonic blastomeres

Shawn Chavez, Sohyun L. McElroy, Nancy L. Bossert, Christopher J. De Jonge, Maria Vera Rodriguez, Denise E. Leong, Barry Behr, Lynn M. Westphal, Renee A. Reijo Pera

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

A map of human embryo development that combines imaging, molecular, genetic and epigenetic data for comparisons to other species and across pathologies would be greatly beneficial for basic science and clinical applications. Here, we compared mRNA and protein expression of key mediators of DNA methylation and histone modifications between mouse and human embryos, embryos from fertile/infertile couples, and following growth factor supplementation. We observed that individual mouse and human embryos are characterized by similarities and distinct differences in DNA methylation and histone modification patterns especially at the single-cell level. In particular, while mouse embryos first exhibited sub-compartmentalization of different histone modifications between blastomeres at the morula stage and cell sub-populations in blastocysts, differential histone modification expression was detected between blastomeres earlier in human embryos at the four- to eight-cell stage. Likewise, differences in epigenetic mediator expression were also observed between embryos from fertile and infertile couples, which were largely equalized in response to growth factor supplementation, suggesting that select growth factors might prevent alterations in epigenetic profiles during prolonged embryo culture. Finally, we determined that reduced expression via morpholino technologies of a single histone-modifying enzyme, Rps6ka4/Msk2, resulted in cleavage-stage arrest as assessed by time-lapse imaging and was associated with aneuploidy generation. Taken together, data document differences in epigenetic patterns between species with implications for fertility and suggest functional roles for individual epigenetic factors during pre-implantation development.

Original languageEnglish (US)
Article numberddu212
Pages (from-to)4970-4984
Number of pages15
JournalHuman Molecular Genetics
Volume23
Issue number18
DOIs
StatePublished - 2014
Externally publishedYes

Fingerprint

Blastomeres
Epigenomics
Histone Code
Embryonic Structures
Intercellular Signaling Peptides and Proteins
DNA Methylation
Time-Lapse Imaging
Morula
Morpholinos
Human Development
Blastocyst
Aneuploidy
Histones
Embryonic Development
Fertility
Molecular Biology
Pathology
Technology
Messenger RNA
Enzymes

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)
  • Molecular Biology

Cite this

Chavez, S., McElroy, S. L., Bossert, N. L., De Jonge, C. J., Rodriguez, M. V., Leong, D. E., ... Reijo Pera, R. A. (2014). Comparison of epigenetic mediator expression and function in mouse and human embryonic blastomeres. Human Molecular Genetics, 23(18), 4970-4984. [ddu212]. https://doi.org/10.1093/hmg/ddu212

Comparison of epigenetic mediator expression and function in mouse and human embryonic blastomeres. / Chavez, Shawn; McElroy, Sohyun L.; Bossert, Nancy L.; De Jonge, Christopher J.; Rodriguez, Maria Vera; Leong, Denise E.; Behr, Barry; Westphal, Lynn M.; Reijo Pera, Renee A.

In: Human Molecular Genetics, Vol. 23, No. 18, ddu212, 2014, p. 4970-4984.

Research output: Contribution to journalArticle

Chavez, S, McElroy, SL, Bossert, NL, De Jonge, CJ, Rodriguez, MV, Leong, DE, Behr, B, Westphal, LM & Reijo Pera, RA 2014, 'Comparison of epigenetic mediator expression and function in mouse and human embryonic blastomeres', Human Molecular Genetics, vol. 23, no. 18, ddu212, pp. 4970-4984. https://doi.org/10.1093/hmg/ddu212
Chavez, Shawn ; McElroy, Sohyun L. ; Bossert, Nancy L. ; De Jonge, Christopher J. ; Rodriguez, Maria Vera ; Leong, Denise E. ; Behr, Barry ; Westphal, Lynn M. ; Reijo Pera, Renee A. / Comparison of epigenetic mediator expression and function in mouse and human embryonic blastomeres. In: Human Molecular Genetics. 2014 ; Vol. 23, No. 18. pp. 4970-4984.
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