Comparison of anterior segment structures in two rat glaucoma models

An ultrasound biomicroscopic study

Nikolaos Nissirios, Raul Chanis, Elaine Johnson, John Morrison, William O. Cepurna, Lijun Jia, Thomas Mittag, John Danias

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

PURPOSE. Optic nerve disease in chronic IOP elevation rat glaucoma models develops at different rates. This study was undertaken to investigate whether anterior chamber (AC) changes develop in two popular models in vivo and whether the changes are related to IOP. METHODS. Ten female Wistar rats and 12 male Brown-Norway rats were subjected to episcleral vein cauterization (EVC) and hypertonic saline episcleral vein sclerosis (HSEVS), respectively. Contralateral untreated eyes served as controls. IOP was recorded for a period of 5 to 6 weeks, and with the rats under anesthesia, the eyes were imaged with an ultrasound biomicroscope. Measurements of the AC depth (ACD), trabecular-iris angle (TIA), iris thickness at the thickest point near the pupillary margin (IT), angle opening distance (AOD; at 200 μm from the scleral spur), and ciliary body area (CBA) were compared between control eyes of the two strains and between experimental and control eyes within each strain. The differences were correlated with IOP history. RESULTS. Eyes subjected to EVC demonstrated greater increases in IOP than eyes subjected to HSEVS. Between rat strains, control eyes differed significantly in all the parameters studied, except for ACD. No difference was detected between experimental and control eyes in the EVC group. In contrast, experimental eyes in the HSEVS group had ∼71% larger ACDs and ∼32% smaller CBAs than did the contralateral control eyes (P <0.001). ACD and CBA correlated well (R2 = 0.80 and 0.51, respectively) with IOP in the HSEVS group. Two of the experimental eyes in this group showed the presence of ultrasound-scattering material in the AC. CONCLUSIONS. Despite apparently higher IOP exposure, eyes in the EVC rat model of glaucoma do not undergo changes in the AC. In contrast, eyes subjected to HSEVS display deepening of the AC and reduction in size of the ciliary body within 5 to 6 weeks. These changes correlate to IOP exposure and may be the result of specific changes induced by the experimental intervention. These models are likely to rely on different mechanisms of pressure elevation and cannot be used interchangeably.

Original languageEnglish (US)
Pages (from-to)2478-2482
Number of pages5
JournalInvestigative Ophthalmology and Visual Science
Volume49
Issue number6
DOIs
StatePublished - Jun 2008

Fingerprint

Glaucoma
Veins
Anterior Chamber
Sclerosis
Cautery
Ciliary Body
Iris
Optic Nerve Diseases
Wistar Rats
Anesthesia
History
Pressure

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Comparison of anterior segment structures in two rat glaucoma models : An ultrasound biomicroscopic study. / Nissirios, Nikolaos; Chanis, Raul; Johnson, Elaine; Morrison, John; Cepurna, William O.; Jia, Lijun; Mittag, Thomas; Danias, John.

In: Investigative Ophthalmology and Visual Science, Vol. 49, No. 6, 06.2008, p. 2478-2482.

Research output: Contribution to journalArticle

Nissirios, Nikolaos ; Chanis, Raul ; Johnson, Elaine ; Morrison, John ; Cepurna, William O. ; Jia, Lijun ; Mittag, Thomas ; Danias, John. / Comparison of anterior segment structures in two rat glaucoma models : An ultrasound biomicroscopic study. In: Investigative Ophthalmology and Visual Science. 2008 ; Vol. 49, No. 6. pp. 2478-2482.
@article{8880f5e0a3d845e58766550c20e27c3a,
title = "Comparison of anterior segment structures in two rat glaucoma models: An ultrasound biomicroscopic study",
abstract = "PURPOSE. Optic nerve disease in chronic IOP elevation rat glaucoma models develops at different rates. This study was undertaken to investigate whether anterior chamber (AC) changes develop in two popular models in vivo and whether the changes are related to IOP. METHODS. Ten female Wistar rats and 12 male Brown-Norway rats were subjected to episcleral vein cauterization (EVC) and hypertonic saline episcleral vein sclerosis (HSEVS), respectively. Contralateral untreated eyes served as controls. IOP was recorded for a period of 5 to 6 weeks, and with the rats under anesthesia, the eyes were imaged with an ultrasound biomicroscope. Measurements of the AC depth (ACD), trabecular-iris angle (TIA), iris thickness at the thickest point near the pupillary margin (IT), angle opening distance (AOD; at 200 μm from the scleral spur), and ciliary body area (CBA) were compared between control eyes of the two strains and between experimental and control eyes within each strain. The differences were correlated with IOP history. RESULTS. Eyes subjected to EVC demonstrated greater increases in IOP than eyes subjected to HSEVS. Between rat strains, control eyes differed significantly in all the parameters studied, except for ACD. No difference was detected between experimental and control eyes in the EVC group. In contrast, experimental eyes in the HSEVS group had ∼71{\%} larger ACDs and ∼32{\%} smaller CBAs than did the contralateral control eyes (P <0.001). ACD and CBA correlated well (R2 = 0.80 and 0.51, respectively) with IOP in the HSEVS group. Two of the experimental eyes in this group showed the presence of ultrasound-scattering material in the AC. CONCLUSIONS. Despite apparently higher IOP exposure, eyes in the EVC rat model of glaucoma do not undergo changes in the AC. In contrast, eyes subjected to HSEVS display deepening of the AC and reduction in size of the ciliary body within 5 to 6 weeks. These changes correlate to IOP exposure and may be the result of specific changes induced by the experimental intervention. These models are likely to rely on different mechanisms of pressure elevation and cannot be used interchangeably.",
author = "Nikolaos Nissirios and Raul Chanis and Elaine Johnson and John Morrison and Cepurna, {William O.} and Lijun Jia and Thomas Mittag and John Danias",
year = "2008",
month = "6",
doi = "10.1167/iovs.07-0965",
language = "English (US)",
volume = "49",
pages = "2478--2482",
journal = "Investigative Ophthalmology and Visual Science",
issn = "0146-0404",
publisher = "Association for Research in Vision and Ophthalmology Inc.",
number = "6",

}

TY - JOUR

T1 - Comparison of anterior segment structures in two rat glaucoma models

T2 - An ultrasound biomicroscopic study

AU - Nissirios, Nikolaos

AU - Chanis, Raul

AU - Johnson, Elaine

AU - Morrison, John

AU - Cepurna, William O.

AU - Jia, Lijun

AU - Mittag, Thomas

AU - Danias, John

PY - 2008/6

Y1 - 2008/6

N2 - PURPOSE. Optic nerve disease in chronic IOP elevation rat glaucoma models develops at different rates. This study was undertaken to investigate whether anterior chamber (AC) changes develop in two popular models in vivo and whether the changes are related to IOP. METHODS. Ten female Wistar rats and 12 male Brown-Norway rats were subjected to episcleral vein cauterization (EVC) and hypertonic saline episcleral vein sclerosis (HSEVS), respectively. Contralateral untreated eyes served as controls. IOP was recorded for a period of 5 to 6 weeks, and with the rats under anesthesia, the eyes were imaged with an ultrasound biomicroscope. Measurements of the AC depth (ACD), trabecular-iris angle (TIA), iris thickness at the thickest point near the pupillary margin (IT), angle opening distance (AOD; at 200 μm from the scleral spur), and ciliary body area (CBA) were compared between control eyes of the two strains and between experimental and control eyes within each strain. The differences were correlated with IOP history. RESULTS. Eyes subjected to EVC demonstrated greater increases in IOP than eyes subjected to HSEVS. Between rat strains, control eyes differed significantly in all the parameters studied, except for ACD. No difference was detected between experimental and control eyes in the EVC group. In contrast, experimental eyes in the HSEVS group had ∼71% larger ACDs and ∼32% smaller CBAs than did the contralateral control eyes (P <0.001). ACD and CBA correlated well (R2 = 0.80 and 0.51, respectively) with IOP in the HSEVS group. Two of the experimental eyes in this group showed the presence of ultrasound-scattering material in the AC. CONCLUSIONS. Despite apparently higher IOP exposure, eyes in the EVC rat model of glaucoma do not undergo changes in the AC. In contrast, eyes subjected to HSEVS display deepening of the AC and reduction in size of the ciliary body within 5 to 6 weeks. These changes correlate to IOP exposure and may be the result of specific changes induced by the experimental intervention. These models are likely to rely on different mechanisms of pressure elevation and cannot be used interchangeably.

AB - PURPOSE. Optic nerve disease in chronic IOP elevation rat glaucoma models develops at different rates. This study was undertaken to investigate whether anterior chamber (AC) changes develop in two popular models in vivo and whether the changes are related to IOP. METHODS. Ten female Wistar rats and 12 male Brown-Norway rats were subjected to episcleral vein cauterization (EVC) and hypertonic saline episcleral vein sclerosis (HSEVS), respectively. Contralateral untreated eyes served as controls. IOP was recorded for a period of 5 to 6 weeks, and with the rats under anesthesia, the eyes were imaged with an ultrasound biomicroscope. Measurements of the AC depth (ACD), trabecular-iris angle (TIA), iris thickness at the thickest point near the pupillary margin (IT), angle opening distance (AOD; at 200 μm from the scleral spur), and ciliary body area (CBA) were compared between control eyes of the two strains and between experimental and control eyes within each strain. The differences were correlated with IOP history. RESULTS. Eyes subjected to EVC demonstrated greater increases in IOP than eyes subjected to HSEVS. Between rat strains, control eyes differed significantly in all the parameters studied, except for ACD. No difference was detected between experimental and control eyes in the EVC group. In contrast, experimental eyes in the HSEVS group had ∼71% larger ACDs and ∼32% smaller CBAs than did the contralateral control eyes (P <0.001). ACD and CBA correlated well (R2 = 0.80 and 0.51, respectively) with IOP in the HSEVS group. Two of the experimental eyes in this group showed the presence of ultrasound-scattering material in the AC. CONCLUSIONS. Despite apparently higher IOP exposure, eyes in the EVC rat model of glaucoma do not undergo changes in the AC. In contrast, eyes subjected to HSEVS display deepening of the AC and reduction in size of the ciliary body within 5 to 6 weeks. These changes correlate to IOP exposure and may be the result of specific changes induced by the experimental intervention. These models are likely to rely on different mechanisms of pressure elevation and cannot be used interchangeably.

UR - http://www.scopus.com/inward/record.url?scp=47249111635&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=47249111635&partnerID=8YFLogxK

U2 - 10.1167/iovs.07-0965

DO - 10.1167/iovs.07-0965

M3 - Article

VL - 49

SP - 2478

EP - 2482

JO - Investigative Ophthalmology and Visual Science

JF - Investigative Ophthalmology and Visual Science

SN - 0146-0404

IS - 6

ER -