Comparison of ANP binding and sensitivity in brains from hypertensive and normotensive rats

Kevin Grove, Jason Gonçalves, Sylvie Picard, Gaetan Thibault, Christian F. Deschepper

    Research output: Contribution to journalArticle

    1 Citation (Scopus)

    Abstract

    We compared the abundance and sensitivity of atrial natriuretic peptides (ANP) receptors in the brains of spontaneously hypertensive (SHR) and Wistar-Kyoto (WKY) rats and examined the effect of blood pressure on the abundance of brain ANP receptors in several other experimental rat models. Brain slices from SHR generated more guanosine 3′,5′-cyclic monophosphate in response to ANP than brain slices from WKY rats. No differences were found in brain particulate guanylate cyclase activity in both strains of rats. In rat brain homogenates, we observed that ANP bound in a specific and saturable fashion to samples from WKY rats, but not in samples from SHR. In vitro receptor autoradiography revealed that ANP binding was reduced in the subfornical organ, the choroid plexus, and the paraventricular nucleus of SHR compared with WKY rat brains. Correction of hypertension in SHR or induction of hypertension in other strains did not affect ANP binding in any of these brain regions. Altogether, our data suggest that the increased sensitivity of SHR brains to the action of ANP may be a consequence of factors other than the abundance of receptors and that it is not secondary to the elevation of blood pressure.

    Original languageEnglish (US)
    JournalAmerican Journal of Physiology
    Volume272
    Issue number4 PART 2
    StatePublished - 1997

    Fingerprint

    Atrial Natriuretic Factor
    Inbred WKY Rats
    Brain
    Atrial Natriuretic Factor Receptors
    Subfornical Organ
    Blood Pressure
    Hypertension
    Choroid Plexus
    Guanosine
    Paraventricular Hypothalamic Nucleus
    Guanylate Cyclase
    Autoradiography
    Theoretical Models

    Keywords

    • Central nervous system
    • Guanosine 3′,5′-cyclic monophosphate
    • Guanylate cyclase
    • In vitro receptor autoradiography

    ASJC Scopus subject areas

    • Physiology (medical)

    Cite this

    Grove, K., Gonçalves, J., Picard, S., Thibault, G., & Deschepper, C. F. (1997). Comparison of ANP binding and sensitivity in brains from hypertensive and normotensive rats. American Journal of Physiology, 272(4 PART 2).

    Comparison of ANP binding and sensitivity in brains from hypertensive and normotensive rats. / Grove, Kevin; Gonçalves, Jason; Picard, Sylvie; Thibault, Gaetan; Deschepper, Christian F.

    In: American Journal of Physiology, Vol. 272, No. 4 PART 2, 1997.

    Research output: Contribution to journalArticle

    Grove, K, Gonçalves, J, Picard, S, Thibault, G & Deschepper, CF 1997, 'Comparison of ANP binding and sensitivity in brains from hypertensive and normotensive rats', American Journal of Physiology, vol. 272, no. 4 PART 2.
    Grove K, Gonçalves J, Picard S, Thibault G, Deschepper CF. Comparison of ANP binding and sensitivity in brains from hypertensive and normotensive rats. American Journal of Physiology. 1997;272(4 PART 2).
    Grove, Kevin ; Gonçalves, Jason ; Picard, Sylvie ; Thibault, Gaetan ; Deschepper, Christian F. / Comparison of ANP binding and sensitivity in brains from hypertensive and normotensive rats. In: American Journal of Physiology. 1997 ; Vol. 272, No. 4 PART 2.
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