Comparison of ANP binding and sensitivity in brains from hypertensive and normotensive rats

Kevin Grove, Jason Goncalves, Sylvie Picard, Gaetan Thibault, Christian F. Deschepper

Research output: Contribution to journalArticle

Abstract

-We compared the abundance and sensitivity of atrial natriuretic peptides (ANP) receptors in the brains of spontaneously hypertensive (SHR) and Wistar-Kyoto (WKY) rats and examined the effect of blood pressure on the abundance of brain ANP receptors in several other experimental rat models. Brain slices from SHR generated more guanosine 3',5'-cyclic monophosphate in response to ANP than brain slices from WKY rats. No differences were found in brain particulate guanylate cyclase activity in both strains of rats. In rat brain homogenates, we observed that ANP bound in a specific and saturable fashion to samples from WKY rats, but not in samples from SHR. In vitro receptor autoradiography revealed that ANP binding was reduced in the subfornical organ, the choroid plexus, and the paraventricular nucleus of SHR compared with WKY rat brains. Correction of hypertension in SHR or induction of hypertension in other strains did not affect ANP binding in any of these brain regions. Altogether, our data suggest that the increased sensitivity of SHR brains to the action of ANP may be a consequence of factors other'than the abundance of receptors and that it is not secondary to the elevation of blood pressure. guanosine 3',5'-cyclic monophosphate; guanylate cyclase; in vitro receptor autoradiography; central nervous system

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume41
Issue number4
StatePublished - 1997
Externally publishedYes

Fingerprint

Atrial Natriuretic Factor
Inbred WKY Rats
Brain
Atrial Natriuretic Factor Receptors
Guanylate Cyclase
Cyclic GMP
Autoradiography
Subfornical Organ
Blood Pressure
Hypertension
Choroid Plexus
Paraventricular Hypothalamic Nucleus
Theoretical Models
Central Nervous System

ASJC Scopus subject areas

  • Physiology

Cite this

Comparison of ANP binding and sensitivity in brains from hypertensive and normotensive rats. / Grove, Kevin; Goncalves, Jason; Picard, Sylvie; Thibault, Gaetan; Deschepper, Christian F.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 41, No. 4, 1997.

Research output: Contribution to journalArticle

Grove, Kevin ; Goncalves, Jason ; Picard, Sylvie ; Thibault, Gaetan ; Deschepper, Christian F. / Comparison of ANP binding and sensitivity in brains from hypertensive and normotensive rats. In: American Journal of Physiology - Heart and Circulatory Physiology. 1997 ; Vol. 41, No. 4.
@article{a080c19fa62546308823998d3f46b101,
title = "Comparison of ANP binding and sensitivity in brains from hypertensive and normotensive rats",
abstract = "-We compared the abundance and sensitivity of atrial natriuretic peptides (ANP) receptors in the brains of spontaneously hypertensive (SHR) and Wistar-Kyoto (WKY) rats and examined the effect of blood pressure on the abundance of brain ANP receptors in several other experimental rat models. Brain slices from SHR generated more guanosine 3',5'-cyclic monophosphate in response to ANP than brain slices from WKY rats. No differences were found in brain particulate guanylate cyclase activity in both strains of rats. In rat brain homogenates, we observed that ANP bound in a specific and saturable fashion to samples from WKY rats, but not in samples from SHR. In vitro receptor autoradiography revealed that ANP binding was reduced in the subfornical organ, the choroid plexus, and the paraventricular nucleus of SHR compared with WKY rat brains. Correction of hypertension in SHR or induction of hypertension in other strains did not affect ANP binding in any of these brain regions. Altogether, our data suggest that the increased sensitivity of SHR brains to the action of ANP may be a consequence of factors other'than the abundance of receptors and that it is not secondary to the elevation of blood pressure. guanosine 3',5'-cyclic monophosphate; guanylate cyclase; in vitro receptor autoradiography; central nervous system",
author = "Kevin Grove and Jason Goncalves and Sylvie Picard and Gaetan Thibault and Deschepper, {Christian F.}",
year = "1997",
language = "English (US)",
volume = "41",
journal = "American Journal of Physiology - Renal Fluid and Electrolyte Physiology",
issn = "1931-857X",
publisher = "American Physiological Society",
number = "4",

}

TY - JOUR

T1 - Comparison of ANP binding and sensitivity in brains from hypertensive and normotensive rats

AU - Grove, Kevin

AU - Goncalves, Jason

AU - Picard, Sylvie

AU - Thibault, Gaetan

AU - Deschepper, Christian F.

PY - 1997

Y1 - 1997

N2 - -We compared the abundance and sensitivity of atrial natriuretic peptides (ANP) receptors in the brains of spontaneously hypertensive (SHR) and Wistar-Kyoto (WKY) rats and examined the effect of blood pressure on the abundance of brain ANP receptors in several other experimental rat models. Brain slices from SHR generated more guanosine 3',5'-cyclic monophosphate in response to ANP than brain slices from WKY rats. No differences were found in brain particulate guanylate cyclase activity in both strains of rats. In rat brain homogenates, we observed that ANP bound in a specific and saturable fashion to samples from WKY rats, but not in samples from SHR. In vitro receptor autoradiography revealed that ANP binding was reduced in the subfornical organ, the choroid plexus, and the paraventricular nucleus of SHR compared with WKY rat brains. Correction of hypertension in SHR or induction of hypertension in other strains did not affect ANP binding in any of these brain regions. Altogether, our data suggest that the increased sensitivity of SHR brains to the action of ANP may be a consequence of factors other'than the abundance of receptors and that it is not secondary to the elevation of blood pressure. guanosine 3',5'-cyclic monophosphate; guanylate cyclase; in vitro receptor autoradiography; central nervous system

AB - -We compared the abundance and sensitivity of atrial natriuretic peptides (ANP) receptors in the brains of spontaneously hypertensive (SHR) and Wistar-Kyoto (WKY) rats and examined the effect of blood pressure on the abundance of brain ANP receptors in several other experimental rat models. Brain slices from SHR generated more guanosine 3',5'-cyclic monophosphate in response to ANP than brain slices from WKY rats. No differences were found in brain particulate guanylate cyclase activity in both strains of rats. In rat brain homogenates, we observed that ANP bound in a specific and saturable fashion to samples from WKY rats, but not in samples from SHR. In vitro receptor autoradiography revealed that ANP binding was reduced in the subfornical organ, the choroid plexus, and the paraventricular nucleus of SHR compared with WKY rat brains. Correction of hypertension in SHR or induction of hypertension in other strains did not affect ANP binding in any of these brain regions. Altogether, our data suggest that the increased sensitivity of SHR brains to the action of ANP may be a consequence of factors other'than the abundance of receptors and that it is not secondary to the elevation of blood pressure. guanosine 3',5'-cyclic monophosphate; guanylate cyclase; in vitro receptor autoradiography; central nervous system

UR - http://www.scopus.com/inward/record.url?scp=33751323402&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33751323402&partnerID=8YFLogxK

M3 - Article

VL - 41

JO - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

JF - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

SN - 1931-857X

IS - 4

ER -