Abstract
Comparative genomic hybridization produces a map of DNA sequence copy number as a function of chromosomal location throughout the entire genome. Differentially labeled test DNA and normal reference DNA are hybridized simultaneously to normal chromosome spreads. The hybridization is detected with two different fluorochromes. Regions of gain or loss of DNA sequences, such as deletions, duplications, or amplifications, are seen as changes in the ratio of the intensities of the two fluorochromes along the target chromosomes. Analysis of tumor cell lines and primary bladder tumors identified 16 different regions of amplification, many in loci not previously known to be amplified.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 818-821 |
| Number of pages | 4 |
| Journal | Science |
| Volume | 258 |
| Issue number | 5083 |
| State | Published - Oct 30 1992 |
| Externally published | Yes |
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ASJC Scopus subject areas
- General
Cite this
Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors. / Kallioniemi, Anne; Kallioniemi, Olli P.; Sudar, Damir; Rutovitz, Denis; Gray, Joe; Waldman, Fred; Pinkel, Dan.
In: Science, Vol. 258, No. 5083, 30.10.1992, p. 818-821.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors
AU - Kallioniemi, Anne
AU - Kallioniemi, Olli P.
AU - Sudar, Damir
AU - Rutovitz, Denis
AU - Gray, Joe
AU - Waldman, Fred
AU - Pinkel, Dan
PY - 1992/10/30
Y1 - 1992/10/30
N2 - Comparative genomic hybridization produces a map of DNA sequence copy number as a function of chromosomal location throughout the entire genome. Differentially labeled test DNA and normal reference DNA are hybridized simultaneously to normal chromosome spreads. The hybridization is detected with two different fluorochromes. Regions of gain or loss of DNA sequences, such as deletions, duplications, or amplifications, are seen as changes in the ratio of the intensities of the two fluorochromes along the target chromosomes. Analysis of tumor cell lines and primary bladder tumors identified 16 different regions of amplification, many in loci not previously known to be amplified.
AB - Comparative genomic hybridization produces a map of DNA sequence copy number as a function of chromosomal location throughout the entire genome. Differentially labeled test DNA and normal reference DNA are hybridized simultaneously to normal chromosome spreads. The hybridization is detected with two different fluorochromes. Regions of gain or loss of DNA sequences, such as deletions, duplications, or amplifications, are seen as changes in the ratio of the intensities of the two fluorochromes along the target chromosomes. Analysis of tumor cell lines and primary bladder tumors identified 16 different regions of amplification, many in loci not previously known to be amplified.
UR - http://www.scopus.com/inward/record.url?scp=0026495364&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026495364&partnerID=8YFLogxK
M3 - Article
C2 - 1359641
AN - SCOPUS:0026495364
VL - 258
SP - 818
EP - 821
JO - Science
JF - Science
SN - 0036-8075
IS - 5083
ER -