Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors

Anne Kallioniemi, Olli P. Kallioniemi, Damir Sudar, Denis Rutovitz, Joe Gray, Fred Waldman, Dan Pinkel

Research output: Contribution to journalArticle

2728 Citations (Scopus)

Abstract

Comparative genomic hybridization produces a map of DNA sequence copy number as a function of chromosomal location throughout the entire genome. Differentially labeled test DNA and normal reference DNA are hybridized simultaneously to normal chromosome spreads. The hybridization is detected with two different fluorochromes. Regions of gain or loss of DNA sequences, such as deletions, duplications, or amplifications, are seen as changes in the ratio of the intensities of the two fluorochromes along the target chromosomes. Analysis of tumor cell lines and primary bladder tumors identified 16 different regions of amplification, many in loci not previously known to be amplified.

Original languageEnglish (US)
Pages (from-to)818-821
Number of pages4
JournalScience
Volume258
Issue number5083
StatePublished - Oct 30 1992
Externally publishedYes

Fingerprint

Comparative Genomic Hybridization
Cytogenetic Analysis
Fluorescent Dyes
Chromosomes
DNA
Tumor Cell Line
Urinary Bladder Neoplasms
Neoplasms
Genome

ASJC Scopus subject areas

  • General

Cite this

Kallioniemi, A., Kallioniemi, O. P., Sudar, D., Rutovitz, D., Gray, J., Waldman, F., & Pinkel, D. (1992). Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors. Science, 258(5083), 818-821.

Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors. / Kallioniemi, Anne; Kallioniemi, Olli P.; Sudar, Damir; Rutovitz, Denis; Gray, Joe; Waldman, Fred; Pinkel, Dan.

In: Science, Vol. 258, No. 5083, 30.10.1992, p. 818-821.

Research output: Contribution to journalArticle

Kallioniemi, A, Kallioniemi, OP, Sudar, D, Rutovitz, D, Gray, J, Waldman, F & Pinkel, D 1992, 'Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors', Science, vol. 258, no. 5083, pp. 818-821.
Kallioniemi A, Kallioniemi OP, Sudar D, Rutovitz D, Gray J, Waldman F et al. Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors. Science. 1992 Oct 30;258(5083):818-821.
Kallioniemi, Anne ; Kallioniemi, Olli P. ; Sudar, Damir ; Rutovitz, Denis ; Gray, Joe ; Waldman, Fred ; Pinkel, Dan. / Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors. In: Science. 1992 ; Vol. 258, No. 5083. pp. 818-821.
@article{f2216c79999c41a4afd11581505d722a,
title = "Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors",
abstract = "Comparative genomic hybridization produces a map of DNA sequence copy number as a function of chromosomal location throughout the entire genome. Differentially labeled test DNA and normal reference DNA are hybridized simultaneously to normal chromosome spreads. The hybridization is detected with two different fluorochromes. Regions of gain or loss of DNA sequences, such as deletions, duplications, or amplifications, are seen as changes in the ratio of the intensities of the two fluorochromes along the target chromosomes. Analysis of tumor cell lines and primary bladder tumors identified 16 different regions of amplification, many in loci not previously known to be amplified.",
author = "Anne Kallioniemi and Kallioniemi, {Olli P.} and Damir Sudar and Denis Rutovitz and Joe Gray and Fred Waldman and Dan Pinkel",
year = "1992",
month = "10",
day = "30",
language = "English (US)",
volume = "258",
pages = "818--821",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "5083",

}

TY - JOUR

T1 - Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors

AU - Kallioniemi, Anne

AU - Kallioniemi, Olli P.

AU - Sudar, Damir

AU - Rutovitz, Denis

AU - Gray, Joe

AU - Waldman, Fred

AU - Pinkel, Dan

PY - 1992/10/30

Y1 - 1992/10/30

N2 - Comparative genomic hybridization produces a map of DNA sequence copy number as a function of chromosomal location throughout the entire genome. Differentially labeled test DNA and normal reference DNA are hybridized simultaneously to normal chromosome spreads. The hybridization is detected with two different fluorochromes. Regions of gain or loss of DNA sequences, such as deletions, duplications, or amplifications, are seen as changes in the ratio of the intensities of the two fluorochromes along the target chromosomes. Analysis of tumor cell lines and primary bladder tumors identified 16 different regions of amplification, many in loci not previously known to be amplified.

AB - Comparative genomic hybridization produces a map of DNA sequence copy number as a function of chromosomal location throughout the entire genome. Differentially labeled test DNA and normal reference DNA are hybridized simultaneously to normal chromosome spreads. The hybridization is detected with two different fluorochromes. Regions of gain or loss of DNA sequences, such as deletions, duplications, or amplifications, are seen as changes in the ratio of the intensities of the two fluorochromes along the target chromosomes. Analysis of tumor cell lines and primary bladder tumors identified 16 different regions of amplification, many in loci not previously known to be amplified.

UR - http://www.scopus.com/inward/record.url?scp=0026495364&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026495364&partnerID=8YFLogxK

M3 - Article

C2 - 1359641

AN - SCOPUS:0026495364

VL - 258

SP - 818

EP - 821

JO - Science

JF - Science

SN - 0036-8075

IS - 5083

ER -