Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors

Anne Kallioniemi, Olli P. Kallioniemi, Damir Sudar, Denis Rutovitz, Joe W. Gray, Fred Waldman, Dan Pinkel

Research output: Contribution to journalArticle

2754 Scopus citations

Abstract

Comparative genomic hybridization produces a map of DNA sequence copy number as a function of chromosomal location throughout the entire genome. Differentially labeled test DNA and normal reference DNA are hybridized simultaneously to normal chromosome spreads. The hybridization is detected with two different fluorochromes. Regions of gain or loss of DNA sequences, such as deletions, duplications, or amplifications, are seen as changes in the ratio of the intensities of the two fluorochromes along the target chromosomes. Analysis of tumor cell lines and primary bladder tumors identified 16 different regions of amplification, many in loci not previously known to be amplified.

Original languageEnglish (US)
Pages (from-to)818-821
Number of pages4
JournalScience
Volume258
Issue number5083
DOIs
StatePublished - Jan 1 1992

ASJC Scopus subject areas

  • General

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    Kallioniemi, A., Kallioniemi, O. P., Sudar, D., Rutovitz, D., Gray, J. W., Waldman, F., & Pinkel, D. (1992). Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors. Science, 258(5083), 818-821. https://doi.org/10.1126/science.1359641