Comparative genomic hybridization.

S. DeVries, Joe Gray, D. Pinkel, F. M. Waldman, D. Sudar

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Comparative Genomic Hybridization (CGH) is a powerful molecular cytogenetic technique that permits assessment of DNA copy number on a genome-wide scale. Of note, this methodology uses tumor DNA as a probe for fluorescence in situ hybridization (FISH) to normal metaphase chromosomes and does not require dividing cells from the tumor specimen. This unit provides protocols for CGH, for preparation of metaphase chromosomes, tumor and normal DNAs for FISH and for the microscopy and image analysis of CGH experiments.

Original languageEnglish (US)
JournalCurrent protocols in human genetics / editorial board, Jonathan L. Haines ... [et al.]
VolumeChapter 4
StatePublished - May 2001
Externally publishedYes

Fingerprint

Comparative Genomic Hybridization
Metaphase
Fluorescence In Situ Hybridization
DNA
Chromosomes
Neoplasms
Cytogenetic Analysis
Microscopy
Genome

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Comparative genomic hybridization. / DeVries, S.; Gray, Joe; Pinkel, D.; Waldman, F. M.; Sudar, D.

In: Current protocols in human genetics / editorial board, Jonathan L. Haines ... [et al.], Vol. Chapter 4, 05.2001.

Research output: Contribution to journalArticle

@article{80b8e37e8bbb4c8bbfe7fd2e30e54df7,
title = "Comparative genomic hybridization.",
abstract = "Comparative Genomic Hybridization (CGH) is a powerful molecular cytogenetic technique that permits assessment of DNA copy number on a genome-wide scale. Of note, this methodology uses tumor DNA as a probe for fluorescence in situ hybridization (FISH) to normal metaphase chromosomes and does not require dividing cells from the tumor specimen. This unit provides protocols for CGH, for preparation of metaphase chromosomes, tumor and normal DNAs for FISH and for the microscopy and image analysis of CGH experiments.",
author = "S. DeVries and Joe Gray and D. Pinkel and Waldman, {F. M.} and D. Sudar",
year = "2001",
month = "5",
language = "English (US)",
volume = "Chapter 4",
journal = "Current protocols in human genetics / editorial board, Jonathan L. Haines ... [et al.]",
issn = "1934-8266",
publisher = "John Wiley and Sons Inc.",

}

TY - JOUR

T1 - Comparative genomic hybridization.

AU - DeVries, S.

AU - Gray, Joe

AU - Pinkel, D.

AU - Waldman, F. M.

AU - Sudar, D.

PY - 2001/5

Y1 - 2001/5

N2 - Comparative Genomic Hybridization (CGH) is a powerful molecular cytogenetic technique that permits assessment of DNA copy number on a genome-wide scale. Of note, this methodology uses tumor DNA as a probe for fluorescence in situ hybridization (FISH) to normal metaphase chromosomes and does not require dividing cells from the tumor specimen. This unit provides protocols for CGH, for preparation of metaphase chromosomes, tumor and normal DNAs for FISH and for the microscopy and image analysis of CGH experiments.

AB - Comparative Genomic Hybridization (CGH) is a powerful molecular cytogenetic technique that permits assessment of DNA copy number on a genome-wide scale. Of note, this methodology uses tumor DNA as a probe for fluorescence in situ hybridization (FISH) to normal metaphase chromosomes and does not require dividing cells from the tumor specimen. This unit provides protocols for CGH, for preparation of metaphase chromosomes, tumor and normal DNAs for FISH and for the microscopy and image analysis of CGH experiments.

UR - http://www.scopus.com/inward/record.url?scp=44049100714&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=44049100714&partnerID=8YFLogxK

M3 - Article

VL - Chapter 4

JO - Current protocols in human genetics / editorial board, Jonathan L. Haines ... [et al.]

JF - Current protocols in human genetics / editorial board, Jonathan L. Haines ... [et al.]

SN - 1934-8266

ER -