Comparative effects of thermal and surgical trauma on rat muscle protein metabolism

V. L. Mermel, Bruce Wolfe, R. J. Hansen, A. J. Clifford

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A modified intraperitoneal pool flooding technique, employing L-3H-tyrosine, was developed for measuring muscle protein synthetic rates following traumatic injury. Sufficient radiolabeled tyrosine was injected intraperitoneally to effect a six-fold increase in plasma tyrosine concentration (124-800 μM) resulting in constant, sustained specific radioactivities in plasma- and intracellular-free tyrosine pools. Localized vs systemic effects of thermal and surgical trauma on gastrocnemius muscle protein turnover were assessed 2 and 4 days postinjury. Thermal trauma increased total, myofibrillar, and sarcoplasmic muscle protein synthesis (44%) and protein degradation (300%). Conversely, surgical trauma decreased synthesis of total (24%), myofibrillar (14%), and sarcoplasmic (43%) muscle proteins without altering protein degradation. Short-term restriction of pair-fed controls did not affect either aspect of protein turnover.

Original languageEnglish (US)
Pages (from-to)128-136
Number of pages9
JournalJournal of Parenteral and Enteral Nutrition
Volume15
Issue number2
StatePublished - 1991
Externally publishedYes

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Muscle Proteins
muscle protein
protein metabolism
Tyrosine
tyrosine
Hot Temperature
heat
rats
Wounds and Injuries
protein degradation
Proteolysis
Radioactivity
Skeletal Muscle
protein synthesis
synthesis
Proteins
methodology

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

Cite this

Comparative effects of thermal and surgical trauma on rat muscle protein metabolism. / Mermel, V. L.; Wolfe, Bruce; Hansen, R. J.; Clifford, A. J.

In: Journal of Parenteral and Enteral Nutrition, Vol. 15, No. 2, 1991, p. 128-136.

Research output: Contribution to journalArticle

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