Compact myelin dictates the differential targeting of two sodium channel isoforms in the same axon

Tatiana Boiko, Matthew N. Rasband, S. Rock Levinson, John H. Caldwell, Gail Mandel, James S. Trimmer, Gary Matthews

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Abstract

Voltage-dependent sodium channels are uniformly distributed along unmyelinated axons, but are highly concentrated at nodes of Ranvier in myelinated axons. Here, we show that this pattern is associated with differential localization of distinct sodium channel α subunits to the unmyelinated and myelinated zones of the same retinal ganglion cell axons. In adult axons, Nav1.2 is localized to the unmyelinated zone, whereas Nav1.6 is specifically targeted to nodes. During development, Nav1.2 is expressed first and becomes clustered at immature nodes of Ranvier, but as myelination proceeds, Nav1.6 replaces Nav1.2 at nodes. In Shiverer mice, which lack compact myelin, Nav1.2 is found throughout adult axons, whereas little Nav1.6 is detected. Together, these data show that sodium channel isoforms are differentially targeted to distinct domains of the same axon in a process associated with formation of compact myelin.

Original languageEnglish (US)
Pages (from-to)91-104
Number of pages14
JournalNeuron
Volume30
Issue number1
DOIs
Publication statusPublished - Apr 2001
Externally publishedYes

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ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Boiko, T., Rasband, M. N., Levinson, S. R., Caldwell, J. H., Mandel, G., Trimmer, J. S., & Matthews, G. (2001). Compact myelin dictates the differential targeting of two sodium channel isoforms in the same axon. Neuron, 30(1), 91-104. https://doi.org/10.1016/S0896-6273(01)00265-3