Abstract
The combining site specificities of eight monoclonal antibodies raised against the organophosphorous-containing hapten Soman are compared to monoclonal antibodies specific for a naturally occurring organophosphorous compound, phosphocholine (PC). Although these haptens share some structural and spatial features, differences in their chemical structures, most notably the presence or absence of a positive charge, appear to prevent significant cross-reactivity between antibodies specific for each. The murine memory response to PC-KLH has been shown previously to be characterized by the presence of two major groups of antibodies differentiated on the basis of their specificity for free PC and for the nitrophenyl derivative of PC, nitrophenylphosphocholine (NPPC). Interestingly, two groups of hybridoma antibodies were detected in the immune response to Soman-KLH which possess differential specificity for Soman and for a nitrophenyl derivative of Soman.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 401-407 |
| Number of pages | 7 |
| Journal | Molecular Immunology |
| Volume | 24 |
| Issue number | 5 |
| DOIs | |
| State | Published - 1987 |
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ASJC Scopus subject areas
- Molecular Biology
- Immunology
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Combining site specificity of monoclonal antibodies to the organophosphate hapten Soman. / Buenafe, Abigail C.; Rittenberg, Marvin.
In: Molecular Immunology, Vol. 24, No. 5, 1987, p. 401-407.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Combining site specificity of monoclonal antibodies to the organophosphate hapten Soman
AU - Buenafe, Abigail C.
AU - Rittenberg, Marvin
PY - 1987
Y1 - 1987
N2 - The combining site specificities of eight monoclonal antibodies raised against the organophosphorous-containing hapten Soman are compared to monoclonal antibodies specific for a naturally occurring organophosphorous compound, phosphocholine (PC). Although these haptens share some structural and spatial features, differences in their chemical structures, most notably the presence or absence of a positive charge, appear to prevent significant cross-reactivity between antibodies specific for each. The murine memory response to PC-KLH has been shown previously to be characterized by the presence of two major groups of antibodies differentiated on the basis of their specificity for free PC and for the nitrophenyl derivative of PC, nitrophenylphosphocholine (NPPC). Interestingly, two groups of hybridoma antibodies were detected in the immune response to Soman-KLH which possess differential specificity for Soman and for a nitrophenyl derivative of Soman.
AB - The combining site specificities of eight monoclonal antibodies raised against the organophosphorous-containing hapten Soman are compared to monoclonal antibodies specific for a naturally occurring organophosphorous compound, phosphocholine (PC). Although these haptens share some structural and spatial features, differences in their chemical structures, most notably the presence or absence of a positive charge, appear to prevent significant cross-reactivity between antibodies specific for each. The murine memory response to PC-KLH has been shown previously to be characterized by the presence of two major groups of antibodies differentiated on the basis of their specificity for free PC and for the nitrophenyl derivative of PC, nitrophenylphosphocholine (NPPC). Interestingly, two groups of hybridoma antibodies were detected in the immune response to Soman-KLH which possess differential specificity for Soman and for a nitrophenyl derivative of Soman.
UR - http://www.scopus.com/inward/record.url?scp=0023237880&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023237880&partnerID=8YFLogxK
U2 - 10.1016/0161-5890(87)90013-7
DO - 10.1016/0161-5890(87)90013-7
M3 - Article
C2 - 3657786
AN - SCOPUS:0023237880
VL - 24
SP - 401
EP - 407
JO - Molecular Immunology
JF - Molecular Immunology
SN - 0161-5890
IS - 5
ER -