Combining cell type-restricted adenoviral targeting with immunostaining and flow cytometry to identify cells-of-origin of lung cancer

Sarah A. Best, Ariena Kersbergen, Marie-Liesse Labat, Kate D. Sutherland

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Scopus citations

Abstract

Lung cancers display considerable intertumoral heterogeneity, leading to the classification of distinct tumor subtypes. Our understanding of the genetic aberrations that underlie tumor subtypes has been greatly enhanced by recent genomic sequencing studies and state-of-the-art gene targeting technologies, highlighting evidence that distinct lung cancer subtypes may be derived from different “cells-of-origin”. Here, we describe the intra-tracheal delivery of cell type-restricted Ad5-Cre viruses into the lungs of adult mice, combined with immunohistochemical and flow cytometry strategies for the detection of lung cancer-initiating cells in vivo.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages15-29
Number of pages15
DOIs
StatePublished - Jan 1 2018
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1725
ISSN (Print)1064-3745

Keywords

  • Cells-of-origin
  • Flow cytometry
  • Immunohistochemical staining
  • Intra-tracheal injection
  • Recombinant adenovirus
  • Tissue dissociation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Best, S. A., Kersbergen, A., Labat, M-L., & Sutherland, K. D. (2018). Combining cell type-restricted adenoviral targeting with immunostaining and flow cytometry to identify cells-of-origin of lung cancer. In Methods in Molecular Biology (pp. 15-29). (Methods in Molecular Biology; Vol. 1725). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7568-6_2