@inbook{84ee56274c5f4899be5c169239434e3f,
title = "Combining cell type-restricted adenoviral targeting with immunostaining and flow cytometry to identify cells-of-origin of lung cancer",
abstract = "Lung cancers display considerable intertumoral heterogeneity, leading to the classification of distinct tumor subtypes. Our understanding of the genetic aberrations that underlie tumor subtypes has been greatly enhanced by recent genomic sequencing studies and state-of-the-art gene targeting technologies, highlighting evidence that distinct lung cancer subtypes may be derived from different “cells-of-origin”. Here, we describe the intra-tracheal delivery of cell type-restricted Ad5-Cre viruses into the lungs of adult mice, combined with immunohistochemical and flow cytometry strategies for the detection of lung cancer-initiating cells in vivo.",
keywords = "Cells-of-origin, Flow cytometry, Immunohistochemical staining, Intra-tracheal injection, Recombinant adenovirus, Tissue dissociation",
author = "Best, {Sarah A.} and Ariena Kersbergen and Asselin-Labat, {Marie Liesse} and Sutherland, {Kate D.}",
note = "Funding Information: We thank Ellen Tsui, Cary Tsui and Ji-Ying Song for their expert histological assistance. The intra-tracheal procedure is performed by highly-trained staff following protocols approved by the WEHI Animal Ethics Committee. K.D.S. is supported by the Peter and Julie Alston Centenary Fellowship, M.L.A.-L. is the recipient of The Viertel Charitable Foundation Senior Medical Research Fellowship, and S.A.B is supported by the Victorian Cancer Agency Early-Career Fellowship. This work was made possible through Victorian State Government Operational Infrastructure Support and Australian Government NHMRC IRIISS. Publisher Copyright: {\textcopyright} 2018, Springer Science+Business Media, LLC.",
year = "2018",
doi = "10.1007/978-1-4939-7568-6_2",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "15--29",
booktitle = "Methods in Molecular Biology",
}