Colorimetric enzymatic measurement of serum total 3α-hydroxy bile acid concentrations without extraction

M. Y. Qureshi, Stephen Smith, G. M. Murphy

Research output: Contribution to journalArticle

14 Scopus citations


A new method for the simpler colorimetric assay of serum total 3α-hydroxy bile acid concentrations without prior extraction has been investigated. The lowest concentration that could be reliably measured was 3 μmol/l and the results obtained were in agreement with those obtained using extraction and the spectrofluorimetric enzymatic assay (y = 1.007x - 1.86, r = 0.99, n = 36). Results for 20 normal sera collected after an overnight fast were 6.2 ± 5.1 μmol/l (mean ± SD) and in 20 normal plasma randomly collected were 4.9 ± 4.2 μmol/l. This new assay is comparable in specificity and sensitivity to the older spectrofluorimetric enzymatic assays and convenient for routine use.

Original languageEnglish (US)
Pages (from-to)317-320
Number of pages4
JournalJournal of Clinical Pathology
Issue number3
Publication statusPublished - 1984
Externally publishedYes


ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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