Abstract
A new method for the simpler colorimetric assay of serum total 3α-hydroxy bile acid concentrations without prior extraction has been investigated. The lowest concentration that could be reliably measured was 3 μmol/l and the results obtained were in agreement with those obtained using extraction and the spectrofluorimetric enzymatic assay (y = 1.007x - 1.86, r = 0.99, n = 36). Results for 20 normal sera collected after an overnight fast were 6.2 ± 5.1 μmol/l (mean ± SD) and in 20 normal plasma randomly collected were 4.9 ± 4.2 μmol/l. This new assay is comparable in specificity and sensitivity to the older spectrofluorimetric enzymatic assays and convenient for routine use.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 317-320 |
| Number of pages | 4 |
| Journal | Journal of Clinical Pathology |
| Volume | 37 |
| Issue number | 3 |
| State | Published - 1984 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Pathology and Forensic Medicine
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Colorimetric enzymatic measurement of serum total 3α-hydroxy bile acid concentrations without extraction. / Qureshi, M. Y.; Smith, Stephen; Murphy, G. M.
In: Journal of Clinical Pathology, Vol. 37, No. 3, 1984, p. 317-320.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Colorimetric enzymatic measurement of serum total 3α-hydroxy bile acid concentrations without extraction
AU - Qureshi, M. Y.
AU - Smith, Stephen
AU - Murphy, G. M.
PY - 1984
Y1 - 1984
N2 - A new method for the simpler colorimetric assay of serum total 3α-hydroxy bile acid concentrations without prior extraction has been investigated. The lowest concentration that could be reliably measured was 3 μmol/l and the results obtained were in agreement with those obtained using extraction and the spectrofluorimetric enzymatic assay (y = 1.007x - 1.86, r = 0.99, n = 36). Results for 20 normal sera collected after an overnight fast were 6.2 ± 5.1 μmol/l (mean ± SD) and in 20 normal plasma randomly collected were 4.9 ± 4.2 μmol/l. This new assay is comparable in specificity and sensitivity to the older spectrofluorimetric enzymatic assays and convenient for routine use.
AB - A new method for the simpler colorimetric assay of serum total 3α-hydroxy bile acid concentrations without prior extraction has been investigated. The lowest concentration that could be reliably measured was 3 μmol/l and the results obtained were in agreement with those obtained using extraction and the spectrofluorimetric enzymatic assay (y = 1.007x - 1.86, r = 0.99, n = 36). Results for 20 normal sera collected after an overnight fast were 6.2 ± 5.1 μmol/l (mean ± SD) and in 20 normal plasma randomly collected were 4.9 ± 4.2 μmol/l. This new assay is comparable in specificity and sensitivity to the older spectrofluorimetric enzymatic assays and convenient for routine use.
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UR - http://www.scopus.com/inward/citedby.url?scp=0021351413&partnerID=8YFLogxK
M3 - Article
C2 - 6583208
AN - SCOPUS:0021351413
VL - 37
SP - 317
EP - 320
JO - Journal of Clinical Pathology - Clinical Molecular Pathology
JF - Journal of Clinical Pathology - Clinical Molecular Pathology
SN - 0021-9746
IS - 3
ER -