Abstract
A new method for the simpler colorimetric assay of serum total 3α-hydroxy bile acid concentrations without prior extraction has been investigated. The lowest concentration that could be reliably measured was 3 μmol/l and the results obtained were in agreement with those obtained using extraction and the spectrofluorimetric enzymatic assay (y = 1.007x - 1.86, r = 0.99, n = 36). Results for 20 normal sera collected after an overnight fast were 6.2 ± 5.1 μmol/l (mean ± SD) and in 20 normal plasma randomly collected were 4.9 ± 4.2 μmol/l. This new assay is comparable in specificity and sensitivity to the older spectrofluorimetric enzymatic assays and convenient for routine use.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 317-320 |
| Number of pages | 4 |
| Journal | Journal of Clinical Pathology |
| Volume | 37 |
| Issue number | 3 |
| Publication status | Published - 1984 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Pathology and Forensic Medicine
Cite this
Qureshi, M. Y., Smith, S., & Murphy, G. M. (1984). Colorimetric enzymatic measurement of serum total 3α-hydroxy bile acid concentrations without extraction. Journal of Clinical Pathology, 37(3), 317-320.