TY - JOUR
T1 - Colocalization of thrombospondin and syndecan during murine development
AU - Corless, Christopher L.
AU - Mendoza, Arturo
AU - Collins, Tucker
AU - Lawler, Jack
PY - 1992/4
Y1 - 1992/4
N2 - Thrombospondin is an adhesive glycoprotein that is thought to play a role in tissue genesis and repair. We have used a monoclonal anti‐thrombospondin antibody, designated 5G11, to localize thrombospondin in paraformaldehyde fixed, paraffin‐embedded sections of developing mouse embryos. Thrombospondin expression is observed in uterine smooth muscle, endometrial glands, the decidua, and trophoblastic giant cells during the initial phase of post‐implantation development in the embryo. Cardiac myocytes and neuroepithelial cells show positive staining for thrombospondin at day 8.5 of gestation, and this expression continues throughout the development of the myocardium and central nervous system. Strong staining for thrombospondin is seen in developing bone and in the liver. Thrombospondin is also observed in developing smooth muscle and skeletal muscle, as well as in a variety of epithelia, including the epidermis, small intestinal epithelium, lens epithelium, renal tubular epithelium, and the epithelium of the developing tooth. Comparison of thrombospondin staining with that of two known cell surface receptors for thrombospondin, syndecan and the vitronectin receptor, reveals remarkable colocalization of thrombospondin and syndecan in all tissues, but almost no coexpression with the vitronectin receptor. Coexpression of thrombospondin and syndecan may play an important role in cell‐cell or cell‐matrix interactions during development. © 1992 Wiley‐Liss, Inc.
AB - Thrombospondin is an adhesive glycoprotein that is thought to play a role in tissue genesis and repair. We have used a monoclonal anti‐thrombospondin antibody, designated 5G11, to localize thrombospondin in paraformaldehyde fixed, paraffin‐embedded sections of developing mouse embryos. Thrombospondin expression is observed in uterine smooth muscle, endometrial glands, the decidua, and trophoblastic giant cells during the initial phase of post‐implantation development in the embryo. Cardiac myocytes and neuroepithelial cells show positive staining for thrombospondin at day 8.5 of gestation, and this expression continues throughout the development of the myocardium and central nervous system. Strong staining for thrombospondin is seen in developing bone and in the liver. Thrombospondin is also observed in developing smooth muscle and skeletal muscle, as well as in a variety of epithelia, including the epidermis, small intestinal epithelium, lens epithelium, renal tubular epithelium, and the epithelium of the developing tooth. Comparison of thrombospondin staining with that of two known cell surface receptors for thrombospondin, syndecan and the vitronectin receptor, reveals remarkable colocalization of thrombospondin and syndecan in all tissues, but almost no coexpression with the vitronectin receptor. Coexpression of thrombospondin and syndecan may play an important role in cell‐cell or cell‐matrix interactions during development. © 1992 Wiley‐Liss, Inc.
KW - Central nervous system
KW - Mouse embryo
KW - Myocytes
KW - Vitronectin receptor
UR - http://www.scopus.com/inward/record.url?scp=0026724478&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026724478&partnerID=8YFLogxK
U2 - 10.1002/aja.1001930408
DO - 10.1002/aja.1001930408
M3 - Article
C2 - 1380845
AN - SCOPUS:0026724478
SN - 1058-8388
VL - 193
SP - 346
EP - 358
JO - Developmental Dynamics
JF - Developmental Dynamics
IS - 4
ER -