Cognate T Cell Help for CD40-Deficient B Cells Induces c-myc RNA Expression, but DNA Synthesis Requires an Additional Signal Through Surface Ig

Carol E. Schrader, Janet Stavnezer, Hitoshi Kikutani, David Parker

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

To investigate the role of CD40 ligand in the delivery of help to B cells, we examined the Ag-specific interaction of B cells from CD40-deficient mice with a Th2 cell line in vitro. Small resting B cells from normal mice are stimulated to synthesize DNA when they present monovalent Ag (rabbit Fab anti-Ig) to a rabbit Ig-specific Th cell line. This response, which is independent of a signal through the B cell Ag receptor (slg), is nearly absent in B cells from CD40-deficient mice. The CD40-deficient B cells are not defective in Ag presentation because they induce T cell IL-4 synthesis as well as normal B cells. Also, CD40-deficient B cells respond to T cell help with DNA synthesis almost as well as normal B cells if an additional signal is provided through slg. In conjunction with a slg signal, cell contact with helper T cells induces DNA synthesis more effectively than soluble cytokines. CD40-independent T cell help can also be measured as an early increase in c-myc mRNA levels in CD40-deficient B cells presenting Ag to helper T cells, although the levels of c-myc RNA expression are lower than those in normal B cells. However, c-myc RNA induced by noncognate interaction with anti-CD3-activated T cells is completely CD40 dependent. We conclude that early growth signals from activated Th cells are received by CD40-/- B cells, but that CD40 and/or slg signals are required for efficient induction of DNA synthesis.

Original languageEnglish (US)
Pages (from-to)153-162
Number of pages10
JournalJournal of Immunology
Volume158
Issue number1
StatePublished - Jan 1 1997

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B-Lymphocytes
RNA
T-Lymphocytes
DNA
Helper-Inducer T-Lymphocytes
Rabbits
Cell Line
Th2 Cells
CD40 Ligand
Interleukin-4
Cytokines
Messenger RNA
Growth

ASJC Scopus subject areas

  • Immunology

Cite this

Cognate T Cell Help for CD40-Deficient B Cells Induces c-myc RNA Expression, but DNA Synthesis Requires an Additional Signal Through Surface Ig. / Schrader, Carol E.; Stavnezer, Janet; Kikutani, Hitoshi; Parker, David.

In: Journal of Immunology, Vol. 158, No. 1, 01.01.1997, p. 153-162.

Research output: Contribution to journalArticle

Schrader, Carol E. ; Stavnezer, Janet ; Kikutani, Hitoshi ; Parker, David. / Cognate T Cell Help for CD40-Deficient B Cells Induces c-myc RNA Expression, but DNA Synthesis Requires an Additional Signal Through Surface Ig. In: Journal of Immunology. 1997 ; Vol. 158, No. 1. pp. 153-162.
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