Coexpression of the human HLA-A2 or HLA-B7 heavy chain gene and human β2-microglobulin gene in L cells

C. Bernabeu, Richard Maziarz, H. Spits, J. de Vries, S. J. Burakoff, C. Terhorst

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Abstract

L cells expressing human HLA-A2 or HLA-B7 class I antigen heavy chains are not recognized by human cytotoxic T lymphocytes directed at HLA-A2 or HLA-B7 antigens. To test whether the absence of human β2-m was the cause of the lack of recognition by the human cytotoxic T lymphocytes, coexpression of the human β2-m gene and the HLA-A2 or HLA-B7 heavy chain in L cells ('double transfectants') was obtained. In addition, L cells expressing HLA-A2 or HLA-B7 antigens in association with human β2-m were obtained by an exchange reaction, in which human β2-m from serum replaced the endogenous murine β2-m. Both types of transfectant cells were used in 51Cr-release assays and cold target inhibition assays for human cytotoxic T cell clones which were directed at HLA-A2 or HLA-B7. Neither human CTL clones nor a mixture of CTL specific for HLA-A2 and HLA-B7 were able to recognize these cells. Several alternative explanations for these observations are discussed.

Original languageEnglish (US)
Pages (from-to)3188-3194
Number of pages7
JournalJournal of Immunology
Volume133
Issue number6
Publication statusPublished - 1984
Externally publishedYes

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ASJC Scopus subject areas

  • Immunology

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Bernabeu, C., Maziarz, R., Spits, H., de Vries, J., Burakoff, S. J., & Terhorst, C. (1984). Coexpression of the human HLA-A2 or HLA-B7 heavy chain gene and human β2-microglobulin gene in L cells. Journal of Immunology, 133(6), 3188-3194.