Coexpression of B7-1 and Antigen Blocks Tolerance Induction to Antigen Presented by Resting B Cells

Victoria N. Yuschenkoff, Michael P. Sethna, Gordon J. Freeman, David C. Parker

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

To investigate a role for B cells as tolerogenic APCs in peripheral lymphoid organs, we have developed a system in which B cells from mice transgenic for the membrane-bound form of human μ-chain are transferred into nontransgenic recipients. Mice injected with B cells expressing human μ-chain became profoundly tolerant to human μ-chain, as shown by greatly reduced Ab responses following challenge with human μ-chain in adjuvant. Adoptive transfer experiments showed that the recipient's Th cell response to human μ-chain was impaired. When the human μ transgenic spleen cells were activated with LPS before transfer, they no longer induced tolerance. Spleen cells from double-transgenic mice expressing both human μ-chain and the costimulatory molecule B7-1 (CD80) also failed to induce tolerance to human μ-chain. However, neither human μ transgenic LPS blasts nor double-transgenic B cells induced an Ab response or primed for a secondary Ab response to Ag in adjuvant. Therefore, we find that expression of B7-1 together with Ag can interfere with tolerance induction without inducing Ab formation or priming for a secondary Ab response.

Original languageEnglish (US)
Pages (from-to)1987-1995
Number of pages9
JournalJournal of Immunology
Volume157
Issue number5
StatePublished - Sep 1 1996

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Yuschenkoff, V. N., Sethna, M. P., Freeman, G. J., & Parker, D. C. (1996). Coexpression of B7-1 and Antigen Blocks Tolerance Induction to Antigen Presented by Resting B Cells. Journal of Immunology, 157(5), 1987-1995.