To investigate a role for B cells as tolerogenic APCs in peripheral lymphoid organs, we have developed a system in which B cells from mice transgenic for the membrane-bound form of human μ-chain are transferred into nontransgenic recipients. Mice injected with B cells expressing human μ-chain became profoundly tolerant to human μ-chain, as shown by greatly reduced Ab responses following challenge with human μ-chain in adjuvant. Adoptive transfer experiments showed that the recipient's Th cell response to human μ-chain was impaired. When the human μ transgenic spleen cells were activated with LPS before transfer, they no longer induced tolerance. Spleen cells from double-transgenic mice expressing both human μ-chain and the costimulatory molecule B7-1 (CD80) also failed to induce tolerance to human μ-chain. However, neither human μ transgenic LPS blasts nor double-transgenic B cells induced an Ab response or primed for a secondary Ab response to Ag in adjuvant. Therefore, we find that expression of B7-1 together with Ag can interfere with tolerance induction without inducing Ab formation or priming for a secondary Ab response.
|Original language||English (US)|
|Number of pages||9|
|Journal||Journal of Immunology|
|Publication status||Published - Sep 1 1996|
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