Coelomic fluid and blood serum proteins of the metamorphosing bullfrog tadpole

Kent Thornburg, H. H. Hillemann, G. W. Martin

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    Abstract

    Bullfrog (Rana catesbeiana) tadpoles were divided into 4 groups containing Gosner stages as follows: I, 27 to 31; II 32 to 36; III, 37 to 41; IV, 42 to 46. Extractable coelomic fluid volume from late metamorphic tadpoles (Group IV) was found to have a highly significant (p<0.001) negative correlation with stage; tadpole weight also had a negative correlation with stage (p<0.025). No significant correlation was found between coelomic fluid volume and tadpole weight. Group IV blood serum protein levels (x = 1.30 ± SEM 0.10 g/100 ml serum) were shown to be significantly higher than corresponding coelomic fluid serum protein levels (x = 0.80 ± 0.06 g/100 ml). Electrophoresis data demonstrated an increase in the albumin fraction of both blood serum and coelomic fluid during metamorphosis. I 125 labeled human serum albumin equilibrated with the blood serum at equal concentrations about ten hours after an intracoelomic injection. A coelomic fluid turnover time of 26% per hour was calculated from the tracer data.

    Original languageEnglish (US)
    Pages (from-to)249-256
    Number of pages8
    JournalJournal of Experimental Zoology
    Volume193
    Issue number2
    StatePublished - 1975

    Fingerprint

    tadpoles
    blood serum
    blood proteins
    Lithobates catesbeianus
    metamorphosis
    electrophoresis
    albumins
    tracer techniques
    fluids
    injection

    ASJC Scopus subject areas

    • Animal Science and Zoology

    Cite this

    Coelomic fluid and blood serum proteins of the metamorphosing bullfrog tadpole. / Thornburg, Kent; Hillemann, H. H.; Martin, G. W.

    In: Journal of Experimental Zoology, Vol. 193, No. 2, 1975, p. 249-256.

    Research output: Contribution to journalArticle

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