Coculture of monkey ovarian tissue increases survival after vitrification and slow-rate freezing

Richard R. Yeoman, Don P. Wolf, David Lee

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Objective: To assess whether coculture of monkey ovarian tissue after low-temperature storage enhances follicular viability. To assess a novel method of vitrifying ovarian tissue. Design: Prospective in vitro study. Setting: University-affiliated national research center. Animal(s): Ovaries from 15 cynomolgus or rhesus macaques (1-11 years). Intervention(s): Vitrification using a containerless liquid nitrogen emersion system that involves dropping thin cortical pieces suspended in cyroprotectant directly into liquid nitrogen with outcome compared with slow-rate-controlled freezing. Before analysis, some of the thawed tissue was cocultured on mitotically inactivated mouse fetal fibroblast monolayers supplemented with FSH, insulin, transferrin, and selenium. Main Outcome Measure(s): Percentage of oocytes viable using live-dead fluorescent staining with carboxyfluorescein diacetate succinimidyl ester and propidium iodide. Result(s): Postthaw survival rates were 70.4% ± 4.8% of 1,705 follicles after vitrification and 67.3% ± 1.9% of 1,895 follicles after slow-rate freeze in six trials with each method. Coculture of the thawed tissue increased the viability, respectively, to 89% ± 2.1% of 2,833 follicles previously vitrified and to 90.3% ± 1.9% of 2,109 follicles after a slow-rate freeze (P

Original languageEnglish (US)
Pages (from-to)1248-1254
Number of pages7
JournalFertility and Sterility
Volume83
Issue number4 SUPPL.
DOIs
StatePublished - Apr 2005

Fingerprint

Vitrification
Tissue Survival
Coculture Techniques
Freezing
Haplorhini
Nitrogen
Propidium
Transferrin
Selenium
Macaca mulatta
Oocytes
Ovary
Fibroblasts
Outcome Assessment (Health Care)
Insulin
Staining and Labeling
Temperature
Research

Keywords

  • Coculture
  • Follicular viability
  • Ovarian tissue
  • Vitrification

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Coculture of monkey ovarian tissue increases survival after vitrification and slow-rate freezing. / Yeoman, Richard R.; Wolf, Don P.; Lee, David.

In: Fertility and Sterility, Vol. 83, No. 4 SUPPL., 04.2005, p. 1248-1254.

Research output: Contribution to journalArticle

Yeoman, Richard R. ; Wolf, Don P. ; Lee, David. / Coculture of monkey ovarian tissue increases survival after vitrification and slow-rate freezing. In: Fertility and Sterility. 2005 ; Vol. 83, No. 4 SUPPL. pp. 1248-1254.
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