Cocaine- and amphetamine-regulated transcript (CART) peptide

A vasoactive role in the cerebral circulation

Jeffrey Iliff, Nabil Alkayed, Kiarash J. Gloshani, Richard J. Traystman, G. Alexander West

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Cocaine- and amphetamine-regulated transcript (CART) peptides are known to be involved in the stress response and have been implicated in the regulation of the cardiovascular system. We evaluated the direct vasoactive properties of CART in the cerebral circulation and its potential mechanisms of action. Penetrating cerebral arterioles, isolated from male Sprague-Dawley rats, were cannulated using a concentric micropipette setup, pressurized and perfused. The vascular response to intraluminal and extraluminal CART peptide was characterized. The endothelium dependence of this response was assessed by means of the endothelial light-dye injury model. The nonspecific endothelin receptor antagonist PD-145065, the ET(A)-specific antagonist BQ-123, the ET(B)-specific antagonist BQ-788, and the inhibitor of endothelin-converting enzyme phosphoramidon were used to characterize the involvement of the endothelin pathway in the vascular response to CART peptide. Extraluminal and intraluminal application of CART peptide (0.1 nm to 1 μmol/L) evoked a long-lasting dose-dependent constriction of isolated penetrating cerebral arterioles to ∼80% of resting myogenic tone. Disruption of the endothelium by the endothelial light/dye injury model resulted in the abolition of this response (P

Original languageEnglish (US)
Pages (from-to)1376-1385
Number of pages10
JournalJournal of Cerebral Blood Flow and Metabolism
Volume25
Issue number10
DOIs
StatePublished - Oct 2005

Fingerprint

Cerebrovascular Circulation
Amphetamine
Cocaine
Peptides
Arterioles
Endothelium
Blood Vessels
Coloring Agents
Light
Endothelins
Wounds and Injuries
Cardiovascular System
Constriction
Action Potentials
Sprague Dawley Rats
peptide A

Keywords

  • Cerebral blood flow
  • Cocaine- and amphetamine-regulated transcript
  • Endothelin
  • Vasoconstriction

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)
  • Endocrinology, Diabetes and Metabolism

Cite this

Cocaine- and amphetamine-regulated transcript (CART) peptide : A vasoactive role in the cerebral circulation. / Iliff, Jeffrey; Alkayed, Nabil; Gloshani, Kiarash J.; Traystman, Richard J.; West, G. Alexander.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 25, No. 10, 10.2005, p. 1376-1385.

Research output: Contribution to journalArticle

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