Coassembly and coupling of SK2 channels and mGlu5 receptors

Gloria García-Negredo, David Soto, Javier Llorente, Xavier Morató, Koen M.O. Galenkamp, Maricel Gómez-Soler, Víctor Fernández-Dueñas, Masahiko Watanabe, John P. Adelman, Ryuichi Shigemoto, Yugo Fukazawa, Rafael Luján, Francisco Ciruela

Research output: Contribution to journalArticle

9 Scopus citations


Group I metabotropic glutamate (mGlu) receptors regulate hippocampal CA1 pyramidal neuron excitability via Ca2+ wave-dependent activation of small-conductance Ca2+ -activated K+ (SK) channels. Here, we show that mGlu5 receptors and SK2 channels coassemble in heterologous coexpression systems and in rat brain. Further, in cotransfected cells or rat primary hippocampal neurons, mGlu5 receptor stimulation activated apamin-sensitive SK2-mediated K+ currents. In addition, coexpression of mGlu5 receptors and SK2 channels promoted plasma membrane targeting of both proteins and correlated with increased mGlu5 receptor function that was unexpectedly blocked by apamin. These results demonstrate a reciprocal functional interaction between mGlu5 receptors and SK2 channels that reflects their molecular coassembly.

Original languageEnglish (US)
Pages (from-to)14793-14802
Number of pages10
JournalJournal of Neuroscience
Issue number44
StatePublished - Oct 29 2014


  • Apamin
  • Electron microscopy
  • Oligomerization
  • SK2 channel
  • mGlu receptor

ASJC Scopus subject areas

  • Neuroscience(all)

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    García-Negredo, G., Soto, D., Llorente, J., Morató, X., Galenkamp, K. M. O., Gómez-Soler, M., Fernández-Dueñas, V., Watanabe, M., Adelman, J. P., Shigemoto, R., Fukazawa, Y., Luján, R., & Ciruela, F. (2014). Coassembly and coupling of SK2 channels and mGlu5 receptors. Journal of Neuroscience, 34(44), 14793-14802.