Coassembly and coupling of SK2 channels and mGlu5 receptors

Gloria García-Negredo, David Soto, Javier Llorente, Xavier Morató, Koen M O Galenkamp, Maricel Gómez-Soler, Víctor Fernández-Dueñas, Masahiko Watanabe, John Adelman, Ryuichi Shigemoto, Yugo Fukazawa, Rafael Luján, Francisco Ciruela

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Group I metabotropic glutamate (mGlu) receptors regulate hippocampal CA1 pyramidal neuron excitability via Ca2+ wave-dependent activation of small-conductance Ca2+ -activated K+ (SK) channels. Here, we show that mGlu5 receptors and SK2 channels coassemble in heterologous coexpression systems and in rat brain. Further, in cotransfected cells or rat primary hippocampal neurons, mGlu5 receptor stimulation activated apamin-sensitive SK2-mediated K+ currents. In addition, coexpression of mGlu5 receptors and SK2 channels promoted plasma membrane targeting of both proteins and correlated with increased mGlu5 receptor function that was unexpectedly blocked by apamin. These results demonstrate a reciprocal functional interaction between mGlu5 receptors and SK2 channels that reflects their molecular coassembly.

Original languageEnglish (US)
Pages (from-to)14793-14802
Number of pages10
JournalJournal of Neuroscience
Volume34
Issue number44
DOIs
StatePublished - Oct 29 2014

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Apamin
Calcium-Activated Potassium Channels
Metabotropic Glutamate Receptors
Pyramidal Cells
Protein Transport
Cell Membrane
Neurons
Brain

Keywords

  • Apamin
  • Electron microscopy
  • mGlu receptor
  • Oligomerization
  • SK2 channel

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)

Cite this

García-Negredo, G., Soto, D., Llorente, J., Morató, X., Galenkamp, K. M. O., Gómez-Soler, M., ... Ciruela, F. (2014). Coassembly and coupling of SK2 channels and mGlu5 receptors. Journal of Neuroscience, 34(44), 14793-14802. https://doi.org/10.1523/JNEUROSCI.2038-14.2014

Coassembly and coupling of SK2 channels and mGlu5 receptors. / García-Negredo, Gloria; Soto, David; Llorente, Javier; Morató, Xavier; Galenkamp, Koen M O; Gómez-Soler, Maricel; Fernández-Dueñas, Víctor; Watanabe, Masahiko; Adelman, John; Shigemoto, Ryuichi; Fukazawa, Yugo; Luján, Rafael; Ciruela, Francisco.

In: Journal of Neuroscience, Vol. 34, No. 44, 29.10.2014, p. 14793-14802.

Research output: Contribution to journalArticle

García-Negredo, G, Soto, D, Llorente, J, Morató, X, Galenkamp, KMO, Gómez-Soler, M, Fernández-Dueñas, V, Watanabe, M, Adelman, J, Shigemoto, R, Fukazawa, Y, Luján, R & Ciruela, F 2014, 'Coassembly and coupling of SK2 channels and mGlu5 receptors', Journal of Neuroscience, vol. 34, no. 44, pp. 14793-14802. https://doi.org/10.1523/JNEUROSCI.2038-14.2014
García-Negredo G, Soto D, Llorente J, Morató X, Galenkamp KMO, Gómez-Soler M et al. Coassembly and coupling of SK2 channels and mGlu5 receptors. Journal of Neuroscience. 2014 Oct 29;34(44):14793-14802. https://doi.org/10.1523/JNEUROSCI.2038-14.2014
García-Negredo, Gloria ; Soto, David ; Llorente, Javier ; Morató, Xavier ; Galenkamp, Koen M O ; Gómez-Soler, Maricel ; Fernández-Dueñas, Víctor ; Watanabe, Masahiko ; Adelman, John ; Shigemoto, Ryuichi ; Fukazawa, Yugo ; Luján, Rafael ; Ciruela, Francisco. / Coassembly and coupling of SK2 channels and mGlu5 receptors. In: Journal of Neuroscience. 2014 ; Vol. 34, No. 44. pp. 14793-14802.
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