Coagulation measurement from whole blood using vibrating optical fiber in a disposable cartridge

Yusuf Samet Yaraş, Ali Bars Gündüz, Gökhan Saǧlam, Selim Ölçer, Fehmi Civitçi, Ibrahim Baris, Göksenin Yaralioǧlu, Hakan Urey

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

In clinics, blood coagulation time measurements are performed using mechanical measurements with blood plasma. Such measurements are challenging to do in a lab-on-a-chip (LoC) system using a small volume of whole blood. Existing LoC systems use indirect measurement principles employing optical or electrochemical methods. We developed an LoC system using mechanical measurements with a small volume of whole blood without requiring sample preparation. The measurement is performed in a microfluidic channel where two fibers are placed inline with a small gap in between. The first fiber operates near its mechanical resonance using remote magnetic actuation and immersed in the sample. The second fiber is a pick-up fiber acting as an optical sensor. The microfluidic channel is engineered innovatively such that the blood does not block the gap between the vibrating fiber and the pick-up fiber, resulting in high signal-to-noise ratio optical output. The control plasma test results matched well with the plasma manufacturer's datasheet. Activated-partial-thromboplastin-time tests were successfully performed also with human whole blood samples, and the method is proven to be effective. Simplicity of the cartridge design and cost of readily available materials enable a low-cost point-of-care device for blood coagulation measurements.

Original languageEnglish (US)
Article number117001
JournalJournal of biomedical optics
Volume22
Issue number11
DOIs
StatePublished - Nov 2017
Externally publishedYes

Keywords

  • activated partial thromboplastin time
  • blood coagulation
  • fiber optic sensor
  • lab-on-a-chip
  • point-of-care

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

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