Co-localization of mu opioid receptor and N-methyl-D-aspartate receptor in the trigeminal dorsal horn

Sue Aicher, Alla Goldberg, Sarita Sharma

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Antagonists acting at the N-methyl-D-aspartate (NMDA) receptor can block the development of tolerance to the analgesic effects of μ opioid receptor (MOR) ligands, such as morphine, and can also enhance the analgesic efficacy of opioids. These findings have led to the hypothesis that interactions between NMDA receptor and MOR ligands may be due to the co-localization of these receptors on neurons in the dorsal horn. We used dual immunogold and immunoperoxidase immunocytochemistry for MOR1 and NMDAR1 to determine the degree of co-localization of these receptors in neurons of the trigeminal dorsal horn. By use of electron microscopy, we found that both receptors were primarily located in dendrites and to a lesser extent in perikarya, axons, axon terminals, and glia. With regard to the degree of co-localization in dendrites, 63% of MOR1-labeled dendrites also contained NMDAR1, whereas 61% of NMDAR1-labeled dendrites also contained MOR1. Most of the dual-labeled profiles (94%) were classified as dendrites, with the remainder being axons, axon terminals, or perikarya. These results suggest that direct interactions between MOR and NMDA receptor ligands are likely mediated through shared dendritic targets in the dorsal horn. Less frequently, we found evidence for modulation of afferents to MOR-containing neurons through presynaptic NMDA receptors.

Original languageEnglish (US)
Pages (from-to)203-210
Number of pages8
JournalJournal of Pain
Volume3
Issue number3
DOIs
StatePublished - 2002

Fingerprint

mu Opioid Receptor
Dendrites
N-Methyl-D-Aspartate Receptors
Posterior Horn Cells
Presynaptic Terminals
Ligands
Axons
Opioid Receptors
Neuroglia
Morphine
Opioid Analgesics
Analgesics
Electron Microscopy
Immunohistochemistry
Spinal Cord Dorsal Horn
Neurons
NMDA receptor A1

Keywords

  • Electron microscopy
  • Glutamate receptor
  • Immunocytochemistry
  • Nociception
  • Opioid receptors

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine
  • Clinical Neurology
  • Neurology
  • Nursing(all)

Cite this

Co-localization of mu opioid receptor and N-methyl-D-aspartate receptor in the trigeminal dorsal horn. / Aicher, Sue; Goldberg, Alla; Sharma, Sarita.

In: Journal of Pain, Vol. 3, No. 3, 2002, p. 203-210.

Research output: Contribution to journalArticle

@article{c9ff63dbbdd946d2a4d8cff7f0bf9269,
title = "Co-localization of mu opioid receptor and N-methyl-D-aspartate receptor in the trigeminal dorsal horn",
abstract = "Antagonists acting at the N-methyl-D-aspartate (NMDA) receptor can block the development of tolerance to the analgesic effects of μ opioid receptor (MOR) ligands, such as morphine, and can also enhance the analgesic efficacy of opioids. These findings have led to the hypothesis that interactions between NMDA receptor and MOR ligands may be due to the co-localization of these receptors on neurons in the dorsal horn. We used dual immunogold and immunoperoxidase immunocytochemistry for MOR1 and NMDAR1 to determine the degree of co-localization of these receptors in neurons of the trigeminal dorsal horn. By use of electron microscopy, we found that both receptors were primarily located in dendrites and to a lesser extent in perikarya, axons, axon terminals, and glia. With regard to the degree of co-localization in dendrites, 63{\%} of MOR1-labeled dendrites also contained NMDAR1, whereas 61{\%} of NMDAR1-labeled dendrites also contained MOR1. Most of the dual-labeled profiles (94{\%}) were classified as dendrites, with the remainder being axons, axon terminals, or perikarya. These results suggest that direct interactions between MOR and NMDA receptor ligands are likely mediated through shared dendritic targets in the dorsal horn. Less frequently, we found evidence for modulation of afferents to MOR-containing neurons through presynaptic NMDA receptors.",
keywords = "Electron microscopy, Glutamate receptor, Immunocytochemistry, Nociception, Opioid receptors",
author = "Sue Aicher and Alla Goldberg and Sarita Sharma",
year = "2002",
doi = "10.1054/jpai.2002.123709",
language = "English (US)",
volume = "3",
pages = "203--210",
journal = "Journal of Pain",
issn = "1526-5900",
publisher = "Churchill Livingstone",
number = "3",

}

TY - JOUR

T1 - Co-localization of mu opioid receptor and N-methyl-D-aspartate receptor in the trigeminal dorsal horn

AU - Aicher, Sue

AU - Goldberg, Alla

AU - Sharma, Sarita

PY - 2002

Y1 - 2002

N2 - Antagonists acting at the N-methyl-D-aspartate (NMDA) receptor can block the development of tolerance to the analgesic effects of μ opioid receptor (MOR) ligands, such as morphine, and can also enhance the analgesic efficacy of opioids. These findings have led to the hypothesis that interactions between NMDA receptor and MOR ligands may be due to the co-localization of these receptors on neurons in the dorsal horn. We used dual immunogold and immunoperoxidase immunocytochemistry for MOR1 and NMDAR1 to determine the degree of co-localization of these receptors in neurons of the trigeminal dorsal horn. By use of electron microscopy, we found that both receptors were primarily located in dendrites and to a lesser extent in perikarya, axons, axon terminals, and glia. With regard to the degree of co-localization in dendrites, 63% of MOR1-labeled dendrites also contained NMDAR1, whereas 61% of NMDAR1-labeled dendrites also contained MOR1. Most of the dual-labeled profiles (94%) were classified as dendrites, with the remainder being axons, axon terminals, or perikarya. These results suggest that direct interactions between MOR and NMDA receptor ligands are likely mediated through shared dendritic targets in the dorsal horn. Less frequently, we found evidence for modulation of afferents to MOR-containing neurons through presynaptic NMDA receptors.

AB - Antagonists acting at the N-methyl-D-aspartate (NMDA) receptor can block the development of tolerance to the analgesic effects of μ opioid receptor (MOR) ligands, such as morphine, and can also enhance the analgesic efficacy of opioids. These findings have led to the hypothesis that interactions between NMDA receptor and MOR ligands may be due to the co-localization of these receptors on neurons in the dorsal horn. We used dual immunogold and immunoperoxidase immunocytochemistry for MOR1 and NMDAR1 to determine the degree of co-localization of these receptors in neurons of the trigeminal dorsal horn. By use of electron microscopy, we found that both receptors were primarily located in dendrites and to a lesser extent in perikarya, axons, axon terminals, and glia. With regard to the degree of co-localization in dendrites, 63% of MOR1-labeled dendrites also contained NMDAR1, whereas 61% of NMDAR1-labeled dendrites also contained MOR1. Most of the dual-labeled profiles (94%) were classified as dendrites, with the remainder being axons, axon terminals, or perikarya. These results suggest that direct interactions between MOR and NMDA receptor ligands are likely mediated through shared dendritic targets in the dorsal horn. Less frequently, we found evidence for modulation of afferents to MOR-containing neurons through presynaptic NMDA receptors.

KW - Electron microscopy

KW - Glutamate receptor

KW - Immunocytochemistry

KW - Nociception

KW - Opioid receptors

UR - http://www.scopus.com/inward/record.url?scp=0036086325&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036086325&partnerID=8YFLogxK

U2 - 10.1054/jpai.2002.123709

DO - 10.1054/jpai.2002.123709

M3 - Article

VL - 3

SP - 203

EP - 210

JO - Journal of Pain

JF - Journal of Pain

SN - 1526-5900

IS - 3

ER -